Thermo Scientific™ Urea Broth

Description

• Differentiate genera within the Enterobacteriaceae on the basis of urease production with Thermo Scientific™ Remel™ Urea Broth (Stuart's)
• Urea Broth (Stuart's) was developed by Stuart et al
• to differentiate Proteus from other genera within the Enterobacteriaceae 1,2
• This medium is designed such that it detects urease activity in organisms which produce ammonia in a highly buffered medium with limited nutrients
• Such organisms include Proteus spp., Morganella morganii , Providencia rettgeri , and urease-positive Providencia stuartii 3
• Urea Broth (Stuart’s) is a liquid medium used to detect microorganisms on the basis of urease production
• It is a medium with high buffering capacity and limited nutrients
• Other genera within the Enterobacteriaceae which hydrolyze urea, will not do so in this medium
• Ready to use – Liquid medium
• Easy to interpret – An intense pink-red color throughout the broth indicates a positive test
• No color change, the broth remains yellow-orange in the case of a negative test
• This medium contains yeast extract that provides essential growth factors required by strong urease-positive organims such as Proteus spp., M
• morganii , and some species of Providencia
• Urea is the substrate that can be hydrolyzed
• Disodium and monopotassium phosphates provide the buffering system
• Phenol red acts as the pH indicator
• Organisms which possess the urease enzyme hydrolyze urea and produce an alkaline by-product (ammonia) which is detected when the phenol red indicator changes color from yellow-orange to pink
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.