Thermo Scientific™ MIO Medium

Description

• Differentiate enteric gram-negative bacilli on the basis of motility, production of indole, and ornithine decarboxylase with Thermo Scientific™ Remel™ MIO Medium (Motility Indole Ornithine Medium)
• In 1970, Ederer and Clark developed MIO Medium (Motility Indole Ornithine Medium) to identify enteric gram-negative bacilli 1
• MIO Medium combines three test into a single tube to facilitate inoculation and identification of Enterobacteriaciae
• Oberhofer and Hajkowski further investigated the use of MIO Medium for non-lactose fermenting, Klebsiella-Enterobacter-Serratia group of organisms 2
• Use MIO Medium for differentiation of gram-negative bacilli on the basis of motility, production of indole, and ornithine decarboxylase
• Easy-to-differentiate: Based on motility, production of indole, and ornithine decarboxylase
• Ready- to-use: Convenience of prepared media
• Enteric gram-negative bacilli ferment dextrose and produce acid that changes the color of brom cresol purple indicator to yellow and also stimulates enzyme activity
• Organisms that possess the enzyme ornithine decarboxylase convert ornithine to putrescine (alkaline) causing the pH indicator to revert to purple
• The enzyme tryptophanase produced by some enteric, gram-negative bacilli degrades tryptophan present in the medium to form indole
• This reaction is detected by adding Kovacs’ reagent to the surface of the medium
• Indole combines with Kovacs’ reagent to form a red complex
• MIO Medium (Motility Indole Ornithine Medium) contains peptone and tryptone that supply nitrogenous compounds and amino acids necessary for the growth of enteric gram-negative bacilli
• Yeast extract acts as the source of B-complex vitamins and dextrose acts as an energy source
• Agar is added to enable detection of motility
• Brom cresol purple is the pH indicator
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.