Thermo Scientific™ Motility B Medium w/TTC

Description

• Demonstrate motility in nonfermentative, gram negative bacilli with Thermo Scientific™ Remel™ Motility B Medium w/TTC Indicator
• Motility B Medium was formulated by Gilardi with a reduced agar concentration to facilitate the appearance of motility by non-fermentative, gram-negative bacilli such as pseudomonads 1,2
• The Gard plate technique that contains Motility B Medium poured into a petri dish 3 is used to select a motile strain
• Kelly and Fulton recommend the addition of 2,3,5-triphenyltetrazolium chloride (TTC) to enhance the macroscopic appearance of motile organisms in motility media 4
• Motility B Medium w/TTC Indicator is a semisolid media that is used to demonstrate motility, primarily in nonfermentative, gram negative bacilli
• Agar provides a semisolid medium which makes motility interpretations macroscopic
• TTC indicator helps to interpret results
• Tetrazolium salt is colorless, but as the organism grows the dye is incorporated into the bacterial cells and is reduced to an insoluble red pigment, formazan
• This red color forms only in the area where bacteria is growing
• Ready- to-use: Convenience of prepared media
• Incubation at room temperature 2 Easy to interpret: Positive test- In presence of TTC, red color is observed in the area of growth
• Negative test- Growth confined to the stab line
• No red color
• Motility B Medium w/TTC Indicator contains casein peptone and yeast extract that supply amino acids, peptides and vitamins to promote growth of nonfermentative gram-negative bacilli
• Sodium chloride provide essential electrolytes and maintains osmotic equilibrium
• 2,3,5-triphenyltetrazolium chloride (TTC) acts as indicator and helps to interpret results by changing color
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.