Thermo Scientific™ MIL Medium (Motility Indole Lysine Medium)

Description

• Differentiate enteric gram-negative bacilli using Thermo Scientific™ Remel™ MIL Medium based on motility, production of indole, lysine deamination and lysine decarboxylation
• MIL Medium was described by Reller and Mirrett in 1975 to facilitate early recognition and presumptive identification of members of the family Enterobacteriaceae 1
• Differentiate enteric gram-negative bacilli based on motility, production of indole, lysine deamination and lysine decarboxylation using MIL Medium
• Easy-to-differentiate: Brom cresol purple is a pH indicator
• Lysine decarboxylase activity shows enteric bacteria producing purple color in the butt of the tube
• Lysine deaminase activity shows a deep red band on the rim of the medium and yellow color in the butt of tube
• Indole activity shows development of pink to red color in the upper layer of the medium after addition of Kovacs reagent
• Ready-to-use : Convenience of prepared media
• This medium contains peptones and tryptone that supply nitrogenous compounds and amino acids necessary for enteric gram-negative bacilli
• Yeast extract is a source of B-complex vitamins and dextrose is an energy source
• Agar is added to enable detection of motility
• Brom cresol purple is a pH indicator that is purple at the initial pH of the medium
• Enteric gram-negative bacilli ferment dextrose, producing acid which changes the pH indicator color to yellow and stimulates enzyme activity
• Lysine is decarboxylated by some enteric bacteria into cadaverine
• This produces a purple butt in the tube because the pH indicator reverts to purple
• Lysine deamination produces α-ketoglutaric acid that results in a deep red rim at the surface of the medium and a yellow butt in the tube
• Indole is produced by organisms that possess the enzyme tryptophanase which degrades tryptophan present in the medium
• This reacts with Kovas reagent and forms a red complex
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.