Thermo Scientific™ PPLO Agar

Description

• Selectively isolate and cultivate Mycoplasma species using Thermo Scientific™ Remel™ PPLO Agar (w/horse serum, amphotericin B, penicillin, and thallium acetate)
• Pleuropneumonia-like organisms (PPLO) were first studied and determined to be the causative agent of bovine pleuropneumonia
• Edward and Freundt proposed the recent system of classification and nomenclature for PPLO, now referred to as Mycoplasmataceae 1,2
• Kraybill confirmed that M
• pneumoniae is resistant to methylene blue in agar medium while other species of Mycoplasma are inhibited 3,4
• The pleuropneumonia-like organisms (PPLO) are a group of pleomorphic, filterable, fastidious microorganisms which also lack a rigid cell wall
• PPLO agar base was formulated to isolate and cultivate Mycoplasma species 5
• Addition of yeast extract and unheated horse serum modified this formulation 6
• Selective medium – Addition of penicillin and thallium acetate in the medium inhibit bacteria, amphotericin B inhibits yeasts and molds and methylene blue inhibits species of Mycoplasma other than pneumoniae
• Ready-to-use – Convenience of prepared medium This medium contains beef extract infusion and peptone which supply nutrients that are necessary for growth of mycoplasmas
• Sodium chloride maintains osmotic equilibrium
• Yeast extract enhances the growth by providing B-complex
• Normal horse serum provides a protein source
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.