Invitrogen™ Terrific Broth

Description

• Terrific Broth is a nutritionally rich bacterial medium for higher density growth and maintenance of recombinant E
• coli strains
• This medium contains increased concentrations of peptone, yeast extract, and glycerol as a carbon source and can be used for nearly all research applications that require a liquid bacterial culturing step such as protein expression, cloning, and expansion
• Terrific Broth was specifically developed to achieve higher plasmid DNA yield from E
• coli and can yield 4–7 times more DNA from pUC-based vectors compared to LB medium
• This versatle medium is also useful for processing a large number of different clones from smaller cultures
• Terrific Broth formulation Terrific Broth contains a higher concentration of yeast extract compared to other media and offers better buffering capacity due higher salt concentrations
• The inclusion of glycerol has a further stabilizing effect on recombinant proteins
• Formulation per liter: • 11.8 g SELECT Peptone 140 • 23.6 g Yeast Extract • 9.4 g dipotassium hydrogen phosphate • 2.2 g potassium dihydrogen phosphate Note: to prepare 1 L of TB media, add 47 g of dehydrated culture medium to 900 mL of distilled water
• Add 4 mL of glycerol (not provided), mix well and add distilled water to 1L
• Adjust the pH value to 7.2 with HCl and NaOH and sterilize by autoclaving for at least 15 minutes at 121°C
• Primary applications • Growth of E
• coli strains to achieve higher plasmid yield • Expression of recombinant proteins using bacteria Find the media format that fits your needs • We offer other ready-to-use media formulations, such as LB Broth (Cat
• No
• 10855021), Terrific Broth (Cat
• No
• A1374301) or S.O.C recovery medium (Cat
• No
• 15544034)
• • UltraPure Glycerol (Cat
• No
• 15514011) is available for cell culture
• Order Info Shipping Condition: Room Temperature