Invitrogen™ 2-YT Broth

Description

• 2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E
• coli and growth of hosts for replication of M13 vectors
• The 2-YT formulation is similar to Lennox LB medium, but contains double the concentration of yeast extract and a 60% higher concentration of peptone
• 2-YT medium allows cells to reach higher density and increase recombinant protein production compared to LB media formulations
• 2-YT Broth formulation Formulation per liter: 16 g SELECT Peptone 140 (pancreatic digest of casein), 10 g SELECT Yeast Extract, and 5 g NaCl
• Note: To prepare 1 L of 2-YT medium, weigh 31 g of powder and add distilled water to 1 L
• Adjust the pH value to 7.0 with 5 N NaOH and sterilize by autoclaving for 20 minutes at 121°C
• Primary applications • Growth of hosts for replication of M13 vectors • Recombinant protein expression • Achieve higher plasmid DNA yield Find the media format that fits you needs We offer a variety of ready-to-use media formulations including LB Broth (Cat
• No
• 10855001), Terrific Broth (Cat
• No
• A1374301) and S.O.C Recovery Medium (Cat
• No
• 15544034)
• Order Info Shipping Condition: Room Temperature