Invitrogen™ LB Agar (Lennox L Agar), powder

Description

• LB Agar (Lennox L agar) is a solid agar media powder recommended for the general maintenance and growth of E
• coli strains
• As E
• coli is unable to digest agar but can absorb nutrition from the LB medium, single colonies can be easily isolated and selected
• With addition of a selective antibiotic, only transformed E
• coli with resistance to that antibiotic can grow
• The Lennox formulation has lower levels of salts than Miller and so is preferred when using salt-sensitive antibiotics such as Zeocin, tetracycline, or chloramphenicol
• Lennox broth is a popular, rich medium used to cultivate bacteria and to perform coliphage plaque assays
• The original formulation was published in 1951 by Guiseppe Bertani in his study of phage plaque formation and named as Lysogeny broth
• It is also known as Lennox broth and Luria-Bertani broth
• Solid culture media have been a major turning point for bacteriology, introduced by Robert Koch in 1881 by adding liquid cultures in gelatin (later replaced by agar derived from seaweed as per Walter Hesse's discovery)
• This allowed plated bacteria to form visible colonies on a solid and smooth surface
• Koch’s assistant, Julius Petri, designed a shallow dish with a lid where medium could be poured, cooled to solidity, and protected from contamination
• Widely used LB medium formulations differ in salt concentration: • Miller: 10 g/L NaCl • Lennox: 5 g/L NaCl • Luria: 0.5 g/L NaCl LB Agar (Lennox L agar) formulation and preparation Formulation per liter: 10 g SELECT Peptone 140, 5 g SELECT Yeast Extract, 5 g NaCl, and 12 g SELECT Agar
• To prepare 1 L of LB Agar medium (1.2% final agar concentration), weigh 37 g of powder and add distilled water to 1 L
• Adjust the pH value to 7.0 with 5 N NaOH and sterilize by autoclaving for 25 minutes at 121°C
• Primary applications • Select using antibiotic transformed E
• coli colony with constructed DNA plasmid • Streaking and isolating bacteria cells on LB agar plate • Blue-white screening using X-Gal or Bluo-gal and IPTG LB agar plates Find the media format that you need • We offer other ready-to-use media formulations, such as LB (Lennox) (Cat
• No
• 10855001), Terrific Broth (Cat
• No
• A1374301) or S.O.C recovery medium (Cat
• No
• 15544034)
• • Explore our media additives: X-Gal (Cat
• No
• 15520034), Bluo-gal (Cat
• No
• 15519028), IPTG (Cat
• No
• 15529019)
• Order Info Shipping Condition: Room Temperature