Invitrogen™ LB Broth Base (Lennox)

Description

• LB Broth Base (Lennox formulation) is a popular, rich medium used to cultivate bacteria and perform coliphage plaque assays
• The original formulation was published in 1951 by Guiseppe Bertani in his study of phage plaque formation and named Lysogeny broth
• It is also known as Lennox broth and Luria-Bertani broth
• Widely used LB medium formulations differ in salt concentration: • Miller: 10 g/L NaCl • Lennox: 5 g/L NaCl • Luria: 0.5 g/L NaCl LB Broth Base is recommended for the general maintenance and growth of E
• coli as it allows for fast cell expansion and reach high cell density
• Its Lennox formulation has lower levels of salts than Miller and is recommended to use with salt-sensitive antibiotics such as Zeocin, tetracycline, or chloramphenicol
• LB Broth Base formulation and preparation To prepare 1 L of Lennox-LB media, weigh 20 g of powder, and add distilled water to 1 L
• Adjust the pH value to 7.0 with 5 N NaOH and sterilize by autoclaving for 25 minutes at 121°C
• Formulation per liter: 10 g SELECT Peptone 140, 5 g SELECT Yeast Extract, and 5 g NaCl
• Primary applications • Growth and maintenance of E
• coli strains for plasmid purification and/or protein expression • Resuspension of T
• pyriformis cell fractions Find the media format that fits your needs We offer other ready-to-use media formulations, such as LB (Lennox) (Cat
• No
• 10855001), Terrific Broth (Cat
• No
• A1374301) or S.O.C recovery medium (Cat
• No
• 15544034)
• Order Info Shipping Condition: Room Temperature