PI15156
Thermo Scientific™ Pierce™ Protein Coated Plates
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Pack of 5 | PI15156-Pack-of-5 | In Stock | ₹ 32,641.14 |
PI15156 - Pack of 5
In Stock
Quantity
1
Base Price: ₹ 32,641.14
GST (18%): ₹ 5,875.405
Total Price: ₹ 38,516.545
Binding Property
Standard Capacity, Affinity
Description
White, 96-well
Product Line
Pierce
Product Type
Microplate
No. of Wells
96
No. per Pack
5 Plates
Content And Storage
Note: Plates are activated to 100 μL and supplied pre-blocked with Thermo Scientific SuperBlock Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.
Detection Method
Chemiluminescence
Color
White
Material
Polystyrene
Surface Treatment
Protein G
Plate Blocking
SuperBlock™ Buffer
Related Products
Description
- These plates are uniformly and stably coated with one of four popular immunoglobilin-binding proteins (Protein A, Protein G, Protein A/G, or Protein L)
- They enable antibodies, or pre-established antibody-antigen complexes, to be captured to wells of the polystyrene plate for subsequent detection in plate readers
- The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA
- Thermo Scientific Pierce Protein Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques
- Highlights • Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption • Orient antibodies for maximum antigen-binding capacity • Immobilize antibodies for plate assays without prior purification • Ensure minimal variation (<5% well-to-well) from consistent coating • Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer • Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG) Protein A/G Coated Plates • Fusion protein containing four antibody-binding sites from Protein A and two from Protein G • Binds to most IgG species because it combines the specificities of Protein A and Protein G • Binds to Fc region of antibodies, ensuring optimal orientation • Binds well using a wide range of pH Protein A Coated Plates • Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey • Binds strongly to mouse IgG2a, IgG2b and IgG3 • Binds to Fc region of antibodies for optimal orientation Protein G Coated Plates • Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat • Binds only to IgG—no cross-reactivity with other antibody classes • Binds to Fc region of antibodies for optimal orientation Protein L Coated Plates • Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD) • Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites • Binds to Fab fragments and ScFv • Does not bind bovine, goat or sheep immunoglobulin • Binds weakly to rabbit immunoglobulin • Pre-blocked to reduce nonspecific binding
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Binding Property: Standard Capacity, Affinity
Description: White, 96-well
Product Line: Pierce
Product Type: Microplate
No. of Wells: 96
No. per Pack: 5 Plates
Content And Storage: Note: Plates are activated to 100 μL and supplied pre-blocked with Thermo Scientific SuperBlock Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.
Detection Method: Chemiluminescence
Color: White
Material: Polystyrene
Surface Treatment: Protein G
Plate Blocking: SuperBlock™ Buffer
Binding Property:
Standard Capacity, Affinity
Description:
White, 96-well
Product Line:
Pierce
Product Type:
Microplate
No. of Wells:
96
No. per Pack:
5 Plates
Content And Storage:
Note: Plates are activated to 100 μL and supplied pre-blocked with Thermo Scientific SuperBlock Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.
Detection Method:
Chemiluminescence
Color:
White
Material:
Polystyrene
Surface Treatment:
Protein G
Plate Blocking:
SuperBlock™ Buffer
Binding Property: Standard Capacity, Affinity
Description: Black, 96-well
Product Line: Pierce
Product Type: Microplate
No. of Wells: 96
No. per Pack: 5 Plates
Content And Storage: Note: Plates are activated to 100 μL and supplied pre-blocked with Thermo Scientific SuperBlock Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.
Detection Method: Fluorescence
Color: Black
Material: Polystyrene
Surface Treatment: Protein G
Plate Blocking: SuperBlock™ Buffer
Binding Property:
Standard Capacity, Affinity
Description:
Black, 96-well
Product Line:
Pierce
Product Type:
Microplate
No. of Wells:
96
No. per Pack:
5 Plates
Content And Storage:
Note: Plates are activated to 100 μL and supplied pre-blocked with Thermo Scientific SuperBlock Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.
Detection Method:
Fluorescence
Color:
Black
Material:
Polystyrene
Surface Treatment:
Protein G
Plate Blocking:
SuperBlock™ Buffer
Binding Property: __
Description: Pierce HisProbe-HRP Conjugate
Product Line: HisProbe™
Product Type: HRP Conjugate
No. of Wells: __
No. per Pack: __
Content And Storage: Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.
Detection Method: Chemiluminescence
Color: __
Material: __
Surface Treatment: __
Plate Blocking: __
Binding Property:
__
Description:
Pierce HisProbe-HRP Conjugate
Product Line:
HisProbe™
Product Type:
HRP Conjugate
No. of Wells:
__
No. per Pack:
__
Content And Storage:
Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.
Detection Method:
Chemiluminescence
Color:
__
Material:
__
Surface Treatment:
__
Plate Blocking:
__
Binding Property: __
Description: Pierce SuperSignal West HisProbe Kit
Product Line: HisProbe™, SuperSignal™
Product Type: HisProbe Detection Kit
No. of Wells: __
No. per Pack: __
Content And Storage: Sufficient For: 62 mini blots or 5000 cm2membrane• HisProbe-HRP Conjugate, 2 mg• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL• SuperSignal West Pico Stable Peroxide Solution, 250 mL• Blocker BSA in TBS (10X), 125 mL• TBS Packs (each makes 500 mL), 10 packs• Surfact-Amps 20 Detergent Solution, 6 x 10 mLStore HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4°C. Store remaining components at 4°C.
Detection Method: Chemiluminescence
Color: __
Material: __
Surface Treatment: __
Plate Blocking: __
Binding Property:
__
Description:
Pierce SuperSignal West HisProbe Kit
Product Line:
HisProbe™, SuperSignal™
Product Type:
HisProbe Detection Kit
No. of Wells:
__
No. per Pack:
__
Content And Storage:
Sufficient For: 62 mini blots or 5000 cm2membrane• HisProbe-HRP Conjugate, 2 mg• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL• SuperSignal West Pico Stable Peroxide Solution, 250 mL• Blocker BSA in TBS (10X), 125 mL• TBS Packs (each makes 500 mL), 10 packs• Surfact-Amps 20 Detergent Solution, 6 x 10 mLStore HisProbe-HRP, Blocker BSA in TBS and Surfact-Amps 20 at 4°C. Store remaining components at 4°C.
Detection Method:
Chemiluminescence
Color:
__
Material:
__
Surface Treatment:
__
Plate Blocking:
__