Description

• General description: DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes. These complexes can be added directly to the cell culture medium. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods.
• Application: The DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. DOTAP can be used for the highly efficient transfection of DNA including yeast artificial chromosomes (YACs) into eukaryotic cells for transient or stable gene expression, and is also suitable for the efficient transfer of other negatively charged molecules, such as RNA, oligonucleotides, nucleotides, ribonucleo-protein (RNP) complexes, and proteins into research samples of mammalian cells.[1][2][3]
• Features and Benefits: Easy to use: The lipid dispersion is simply mixed with the DNA solution, then applied directly to the cellsHighly effective: 5 to 100-fold more effective than the calcium phosphate or DEAE-dextran methodGentle: No cytotoxic effectsFlexible: Equally effective in the presence or absence of serum. Effective on a wide range of species (e.g., insect cells, mammalian cells) and a variety of different cell types. Can be used for either transient or stable transfection procedures.Aqueous dispersion (liposomes) in MBS (MES-buffered saline, pH 6.2) (bottled under argon), 1mg/ml, sterile-filtered.
• Preparation Note: Working concentration: Approximately 5 - 10μg DOTAP/μg DNA, and 10 - 20μg DOTAP/ml cell culture medium.The optimal working concentration is dependent on several parameters, including the cell line being used, concentration and type of nucleic acid (DNA, RNA), and incubation time. It is important to optimize the transfection conditions for the individual cell type studied.Cytotoxicity: Not cytotoxic up to a concentration of 100μg/ml (PBLs, HeLa cells).
• Other Notes: For life science research only. Not for use in diagnostic procedures.