Anti-Digoxigenin-Fluorescein, Fab fragments

Description

• General description: Anti-Digoxigenin-Fluorescein, Fab fragments has fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to fluorescein.
• Specificity: The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
• Application: Anti-Digoxigenin-Fluorescein, Fab fragments are used for the detection of digoxigenin-labeled compounds using:Digoxigenin-labeled sugars in glycoconjugate researchFluorescent in situ hybridization (FISH)[1][2]ImmunohistocytochemistryIn situ hybridization[3]Anti-Digoxigenin-Fluorescein, Fab fragments cannot be used for membrane applications.
• Preparation Note: Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:Detection of digoxigenin-labeled sugars in glycoproteins: 50 to 20μg/mlFluorescent in situ hybridization (FISH): 1 to 20μg/mlImmunohistocytochemistry: 20 to 50μg/mlIn situ hybridization: 20 to 50μg/mlThe anti-DIG anitbody is highly specific and sensitive. Therefore, for FISH, a concentration of 1μg/ml is sufficient, and only in case of critical probes, this concentration may be raised to <10μg/ml (with a distinct background to be expected).Working solution: ReconstitutionAdd 1 ml double-distilled water to a final concentration of 200μg/ml.Blocking:PBS, 0.5% bovine serum albumin (w/v), pH 7.4.1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore, pH can be increased up to pH 8.5 to 9.0.Storage conditions (working solution): Always prepare fresh!
• Reconstitution: Add 1 ml double-distilled water to a final concentration of 200 μg/ml.
• Other Notes: For life science research only. Not for use in diagnostic procedures.