Anti-Digoxigenin

Description

• General description: Contents: Lyophilizate
• Specificity: The monoclonal antibody reacts with free and bound digoxigenin.
• Application: Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using: ELISAImmunohistocytochemistry[3]In situ hybridization[4][5][6]Western blot[3]Immunofluorescence staining[7]FISH (fluorescent in situ hybridization)[8]
• Biochem/physiol Actions: In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.[9]
• Features and Benefits: Specific to free and bound digoxigeninThe antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.
• Preparation Note: Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: ELISA: for coating: 2 to 4 μg/mlImmunohistocytochemistry: 0.5 to 2 μg/mlIn situ hybridization: 0.2 to 0.4 μg/mlWestern blot: 0.5 to 2 μg/mlWorking solution: For coating applicationsphosphate buffered saline, pH 7.4
• Reconstitution: Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
• Storage and Stability: The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.
• Analysis Note: There are no cross reactivities known.
• Other Notes: For life science research only. Not for use in diagnostic procedures.