Anti-Digoxigenin-POD (poly), Fab fragments

Description

• General description: Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.[1] Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.[2] This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.
• Specificity: The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
• Application: Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:whole-mount in situ hybridization[3]fluorescence in situ hybridization[4]enzyme linked immunosorbent assay (ELISA)[5]dot blotSouthern blotwestern blotdouble labeling experimentslectin blotsAnti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.
• Preparation Note: Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Dot blot: 50 to 100 mU/mlELISA: 2 to 50 mU/mlWestern blot: 50 to 100 mU/mlSouthern blot: 50 to 100 mU/mlWorking solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
• Reconstitution: Add 1 ml double-distilled water to a final concentration of 50 U/ml.
• Other Notes: For life science research only. Not for use in diagnostic procedures.