PhasePrep™ BAC DNA Kit

Description

• General description: The PhasePrep™ BAC DNA Kit offers a scalable and cost-effective method for isolating large-molecular weight plasmids such as Bacterial Artificial Chromosomes (BAC) from recombinant E. coli cultures. The same kit can be used for preparations of four different sizes; sufficient reagents are provided for 300 micro, 180 mini, 30 midi, or 15 maxi preparations. Up to 2,12, 50, or 100 mg of BAC DNA can be recovered from 5, 40, 250, or 500 ml of overnight recombinant E. coli culture, respectively. The purified BAC DNA contains very low levels of endotoxin (£10 EU/mg DNA).Recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. Nucleic acid is precipitated from the cleared lysate; residual RNA is removed by a short digestion at elevated temperature with an RNase cocktail. Endotoxins and other impurities are removed by simple temperature-mediated extraction and phase separation. Finally the BAC DNA is selectively precipitated from solution.
• Application: PhasePrep™ BAC DNA Kit has been used to isolate bacterial artificial chromosomes.[1][2][3]
• Features and Benefits: Typical DNA yields of 2-100 μg from 5-500 mL of overnight culturesNo phenol/chloroform extraction requiredAllows possible micro to maxi preps with the same kitNo long waits for drip columns
• Other Notes: The PhasePrep™ BAC DNA Kit offers a highly scalable, rapid, cost-effective method for isolating high molecular weight plasmids such as Bacterial Artificial Chromosomes (BACs) from recombinant E. coli cultures.
• Legal Information: PhasePrep is a trademark of Sigma-Aldrich Co. LLC