SYBR® Green Extract-N-Amp™ Plant PCR Kit

Description

• General description: SYBR® Green Extract-N-Amp™ Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue. The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated. Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
• Features and Benefits: Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes No freezing, mechanical disruption, organic extraction, column purification, or precipitation requiredSpecially formulated PCR ReadyMix for use with extract Hot Start antibody for highly specific PCR amplification of genomic DNA No loading buffers or tracking dyes required for gel analysis SYBR® Green I dye acts as a nonspecific reporter for real-time PCRSYBR® Green I dye is inexpensive, easy to use, and highly sensitive Compatible with high-throughput requirements for genetic analysis of plants No long enzymatic digestions Extract stable at 4°C for at least 6 months
• Other Notes: The protocol uses a 20 μl PCR reaction.
• Legal Information: Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC