First Strand cDNA Synthesis Kit for RT-PCR (AMV)

Description

• General description: The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for two-step RT PCR.The kit includes Reverse Transcriptase AMV for first strand synthesis, two different primers, our PCR Nucleotide Mix, and Control Neo pa RNA. PCR products that are generated by RT-PCR can be cloned using standard procedures.
• Specificity: Heat inactivation: 5 min, 95 °CReverse Transcriptase AMV
• Application: The First Strand cDNA Synthesis Kit for RT-PCR (AMV) is suitable for:Detection of the presence or absence of RNA viruses or other RNA-containing microorganisms (in combination with PCR)Quantification of mRNA for monitoring differential expression of a specific mRNA[1][2]First step in the "differential display of mRNA"[3]Generation of cDNA libraries with large and full-length inserts[4]Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for reverse transcription of RNA into complementary DNA.[5][6][7]
• Features and Benefits: Robustness: Transcribes total RNA, mRNA and viral RNA along with difficult-to-transcribe secondary RNA structures Obtain cDNA transcripts up to 12 kb Higher thermostability (up to 50°C) and specificity than M-MuLV Reverse Transcriptase Purification of the resulting cDNA before PCR reaction is not necessaryFlexibility:It can be used with either sequence-specific primers, poly(dT)15 primers, or random primers, p(dN)6
• Packaging: 1 kit containing 10 components.
• Quality: In the standard cDNA synthesis assay, at least 300 ng of cDNA is synthesized (i.e., 30% yield) when 1.0 μg Neo pa RNA template is incubated with 20 μCi [α-32P]-dCTP (specific activity of 3000 Ci/mmol) for 60 minutes at +42°C.
• Unit Definition: Volume Activity: 20 to 25 U/μl
• Other Notes: For life science research only. Not for use in diagnostic procedures.