JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR

Description

• General description: JumpStart™ Taq ReadyMix™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.To prepare a typical qPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL. Reaction volumes can be scaled down, if desired.
• Application: JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR has been used: For quantitative polymerase chain reaction (qPCR) of cDNA template[1][2]As a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytes[3]For qPCR amplifications using SYBRFor multiplex PCRFor reduction of primer dimers
• Features and Benefits: JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yieldWhen performing large number of PCR reactions, JumpStart™ Taq ReadyMix™ reduces preparation time and the risk of contamination from multiple pipetting steps This master mix allows consistent batch-to-batch and reaction-to-reaction performanceInternal Reference Dye is provided for reaction normalization
• Packaging: Default reaction volume is 50 μL20RXN is packaged as 1 X 500 μL400RXN is packaged as 1 X 10 mL2000RXN is packaged as 1 X 50 mL
• Quantity: Use 2.5 units per reaction.
• Legal Information: Eppendorf is a registered trademark of Eppendorf AG