LuminoCt® SYBR® Green qPCR ReadyMix™
For fast SYBR Green quantitative PCR
Manufacturer: Sigma Aldrich
Synonym(S): LuminoCt®
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 REACTIONS | L6544-100-REACTIONS | In Stock | ₹ 17,406.60 |
| 500 REACTIONS | L6544-500-REACTIONS | In Stock | ₹ 1,01,690.05 |
| 2000 REACTIONS | L6544-2000-REACTIONS | In Stock | ₹ 3,85,651.45 |
L6544 - 100 REACTIONS
In Stock
Quantity
1
Base Price: ₹ 17,406.60
GST (18%): ₹ 3,133.188
Total Price: ₹ 20,539.788
Quality Level
200
form
liquid
usage
sufficient for 100 reactions sufficient for 2000 reactions sufficient for 500 reactions
feature
dNTPs includedhotstart
technique(s)
qPCR: suitable
color
colorless
input
purified DNA
application(s)
agriculture
compatibility
Bio-Rad MyiQ Bio-Rad iCycler iQ Bio-Rad iQ 5for use with ABI 5700for use with ABI 7000for use with ABI 7300for use with ABI 7500 Fastfor use with ABI 7500for use with ABI 7700for use with ABI 7900 Fastfor use with ABI 7900 HTfor use with ABI 7900for use with ABI StepOnefor use with ABI StepOnePlusfor use with ABI ViiA 7for use with Bio-Rad CFX384for use with Bio-Rad CFX96for use with Bio-Rad MJ Chromo4for use with Bio-Rad MJ Opticon 2for use with Bio-Rad MJ Opticon Cepheid SmartCyclerfor use with Bio-Rad MJ Opticonfor use with Bio-Rad MiniOpticonfor use with Eppendorf® Mastercycler ep realplex2 sfor use with Eppendorf® Mastercycler ep realplexfor use with Illumina Eco qPCRfor use with Qiagen Corbett Rotor-Gene 3000for use with Qiagen Corbett Rotor-Gene 6000for use with Qiagen Corbett Rotor-Gene Qfor use with Roche LightCycler 480for use with Strategene Mx3000Pfor use with Strategene Mx3005Pfor use with Strategene Mx4000
detection method
SYBR® Green
Related Products
Description
- General description: LuminoCt® SYBR® Green qPCR ReadyMix is ready to use master mix that combines the performance enhancements of our JumpStart™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assaysKit is not compatible with qPCR instruments that utilize glass capillary tubes
- Application: LuminoCt® SYBR® Green qPCR ReadyMix™ has been used for fast quantitative polymerase chain reaction (qPCR) and quantitative RT-PCR amplifications.[1][2][3][4][5] It has also been used in qPCR for measuring gene expression.[6]
- Features and Benefits: Ideal for performing high-throughput, quantitative PCR applications The master mix allows consistency from one reaction to the next Reduced preparation time and reduced risk of contamination from multiple pipetting steps The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results This master mix is compatible with commercial primer sets, including TaqMan® assays
- Packaging: Default reaction volume is 50 μL100RXN is packaged as 1 X 2.5 mL500RXN is packaged as 1 X 12.5 mL2000RXN is packaged as 1 X 50 mL
- Other Notes: At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bpSigma recommends designing primers and probes with Beacon Designer softwareTo order primers and probes from Sigma, visit: www.sigma.com/oligos
- Legal Information: Eppendorf is a registered trademark of Eppendorf AG
SAFETY INFORMATION
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Quality Level: 200
form: liquid
usage: sufficient for 100 reactions sufficient for 2000 reactions sufficient for 500 reactions
feature: dNTPs includedhotstart
technique(s): qPCR: suitable
color: colorless
input: purified DNA
application(s): agriculture
compatibility: Bio-Rad MyiQ Bio-Rad iCycler iQ Bio-Rad iQ 5for use with ABI 5700for use with ABI 7000for use with ABI 7300for use with ABI 7500 Fastfor use with ABI 7500for use with ABI 7700for use with ABI 7900 Fastfor use with ABI 7900 HTfor use with ABI 7900for use with ABI StepOnefor use with ABI StepOnePlusfor use with ABI ViiA 7for use with Bio-Rad CFX384for use with Bio-Rad CFX96for use with Bio-Rad MJ Chromo4for use with Bio-Rad MJ Opticon 2for use with Bio-Rad MJ Opticon Cepheid SmartCyclerfor use with Bio-Rad MJ Opticonfor use with Bio-Rad MiniOpticonfor use with Eppendorf® Mastercycler ep realplex2 sfor use with Eppendorf® Mastercycler ep realplexfor use with Illumina Eco qPCRfor use with Qiagen Corbett Rotor-Gene 3000for use with Qiagen Corbett Rotor-Gene 6000for use with Qiagen Corbett Rotor-Gene Qfor use with Roche LightCycler 480for use with Strategene Mx3000Pfor use with Strategene Mx3005Pfor use with Strategene Mx4000
detection method: SYBR® Green
Quality Level: 200
form: lyophilized powder
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