Quantitative RT-PCR ReadyMix™

Description

• General description: Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.
• Application: Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).[1][2]
• Features and Benefits: The master mix allows consistency and reproducibility from one reaction to the next Reduced risk of contamination from multiple pipetting steps Reduced set-up time as compared to manual or wax Hot Start methods JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation Broad instrument compatibility Includes a separate ROX reference dye vial for reaction normalization
• Packaging: 1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.
• Legal Information: This product is for research use only.