Deoxynucleotide Mix, 10 mM

Description

• General description: Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.
• Application: dNTP Mix has been used: in the PCR amplification of genomic DNA isolated from insect,[1] fungi,[2] virus, human,[3] in reverse transcription of total RNA to cDNA.[4][4] as a component of the DNA amplification mixture for polymerase chain reaction (PCR)[2][5][1]routine and long PCR manual and automated DNA sequencing cDNA synthesis[6] and labeling reactions
• Features and Benefits: Purity of each dNTP: Minimum 99%Conveniently formulated; 1 μL is used per 50 μL PCREquimolar amounts of each dNTP means less pipettingMinimize risk of contamination in PCRUltraPure dNTPs can help maximize consistency and yields in critical PCR reactions