Single-strand Binding Protein from Escherichia coli

Description

• General description: Single-strand Binding Protein is a homotetramer,[1] found in E. coli during the event of DNA metabolism.[2]
• Application: Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.
• Biochem/physiol Actions: Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes.[1][5] Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard.[5] It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication.[5] SSBP mediates the recruitment of other proteins to their sites of function on DNA.[1]
• Physical form: Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol