KAPA Taq EXtra PCR Kit

Description

• Application: The KAPA Taq EXtra PCR system is ideally suited for:PCR of short- and medium-length targets (<5 kb)Long-range PCR (5–15 kb)PCR with limiting amounts of template DNA.
• Biochem/physiol Actions: The KAPA Taq EXtra PCR system is a blend of KAPA Taq DNA polymerase and a modified archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support robust, long range, and sensitive PCR. Both enzymes possess 5′→3′ polymerase activity, but only KAPA Taq DNA polymerase possesses 5′→3′ exonuclease activity and only the Type B DNA polymerase possesses 3′→5′ exonuclease (proofreading) activity. Reaction products generated with KAPA Taq EXtra are polyA-tailed, and can be cloned into TA-cloning vectors.
• Features and Benefits: KAPA Taq EXtra is a blend of KAPA Taq and a proofreading DNA polymerase.Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA.Fidelity is 2-4 times better than TaqFor short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.Amplify mid- to long-range targets with high yield and sensitivity.KAPA Taq EXtra Buffer (Mg-free) is designed for long-range, sensitive PCR. MgCl2 is supplied separately for optimization.The fidelity of KAPA Taq EXtra is 2-4 fold higher than that of KAPA Taq DNA Polymerase, but is lower than the fidelity of pure proofreading polymerases, such as KAPA HiFi.
• Quality: Each batch of KAPA Taq EXtra DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq EXtra PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
• Preparation Note: Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.
• Other Notes: For Research Use Only. Not for use in diagnostic procedures.