KAPA PROBE FAST One-Step

Description

• General description: KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is designed for high-throughput, fast-cycling, one-step RNA quantification using sequence-specific fluorogenic probes. It is compatible with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g. TaqMan®) and displacement probes (e.g., molecular beacons)
• Application: KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal has been used for:Gene expression analysisSNP/mutation analysisGene knockdown validationRT-PCR[1]
• Features and Benefits: Quick Notes:The kit is suitable for all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan) and displacement probes (e.g., molecular beacons).Use only gene-specific primers for one-step qRT-PCR.Optimal cDNA synthesis is achieved at 42°C for 5 min.3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.Do not exceed 25 μL reaction volumes
• Packaging: Kit includes qPCR Master Mix (2X), KAPA RT Mix (50X), dUTP (10 mM), ROX High Reference Dye (50X) and ROX Low Reference Dye (50X)
• Quality: All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination.
• Preparation Note: Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA PROBE FAST qPCR Master Mix may not freeze solidly, even when stored at -20 °C. The KAPA RT Mix is temperature-sensitive, and should be stored at -20 °C and kept on ice during use. ROX reference dye is light-sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
• Other Notes: For Research Use Only. Not for use in diagnostic procedures.
• Legal Information: TaqMan is a registered trademark of Roche Molecular Systems, Inc.