Cytiva Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit

Description

• p> Representative amplification of the whole genome
• Preformulated, predispensed, single-dose, lyophilized cakes for rapid reaction set-up and greater reproducibility
• Quick and simple automation-friendly protocol; no thermal cycler required
• Shipping and storage at room temperature provides easy access and reduced carbon footprint
• Yields high-quality DNA (e.g
• for high-throughput genotyping and DNA archival)
• The reaction mixture is provided as predispensed, single-dose, lyophilized cakes in either strips of 8 tubes, or 96-well plates
• Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample
• Amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity
• The average product length is over 10kb
• Procedure is quick, simple and does not require a thermocycler
• A typical DNA yield of 12 to 20μg DNA can be achieved in less than two hours from only 10ng of genomic DNA input
• The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used
• Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual
• GenomiPhi amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.