QIAGEN QIAcard FTA DMPK

Description

• For collection, stabilization, processing, transport and archiving of biological samples
• 4 sample areas per card
• QIAcard FTA DMPK Cards for Drug Metabolism Pharmacokinetic enable microvolume sampling with only 10 to 20 μL per sample and are designed for use with blood samples
• The cards can also be used with colorless samples such as plasma, cerebrospinal fluid and urine
• QIAcard FTA DMPK-A and QIAcard FTA DMPK-B contains impregnated chemicals enabling protein denaturation to inactivate endogenous enzymes
• Cell lysis releases endogenous cellular materials onto card
• Stabilization of DNA allows resampling of blood spot for pharmacogenomics.Impregnated chemicals may interfere with mass spectrometry detection (e.g
• ion suppression)
• QIAcard FTA DMPK-C contain pure cellulose
• No impregnated chemicals to interfere with analysis
• Proteins are not denatured so cards may be better suited for protein based biomolecules and antibody studies
• Analysis: Analysis primarily with HPLC or UHPLC followed by MS/MS detection, direct desorption MS techniques such as DESI
• Methanol or aqueous/organic mixtures have been shown to work well for small molecules and aqueous buffers are recommended for proteins
• Select a good solvent for the analyte while maintaining compatibility with the HPLC conditions to be used
• The choice of DMPK card depends on many factors such as the analyte chemical structure, extraction solvent and analysis workflow.