12-280-050
Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 12-280-050-Each-of-1 | In Stock | ₹ 29,090.40 |
12-280-050 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 29,090.40
GST (18%): ₹ 5,236.272
Total Price: ₹ 34,326.672
Column Type
Spin Column
Isolation Technology
Silica Spin Column
Scale
Mini
Final Product Type
Viral RNA and DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Yield
5 μg (Binding capacity)
Content And Storage
• 310 μg Carrier RNA; –20°C• 32 mL Viral Lysis Buffer L22; room temperature• 15 mL Wash Buffer WII; room temperature• 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage• 15.5 mL RNase-free Water E3; room temperature• 50 Viral Spin Columns with Collection Tubes; room temperature• 2 x 50 Wash Tubes; room temperature• 50 Recovery Tubes; room temperature
Sample Type
Cells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM)
Elution Volume
10 to 50 μL
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity
50 Preps
Test Time
45 min.
Starting Material Amount
≤500 μL
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Description
- Description The PureLink Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants
- The kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis
- The purified viral RNA/DNA is devoid of proteins and nucleases and is suitable for use in downstream applications that allow viral detection and genotyping
- The PureLink Viral Mini Kit has been used to purify viral dsDNA from orthopoxviruses including cowpox virus, variola virus, monkeypox virus, vaccinia virus, and HERV
- The PureLink Viral RNA/DNA Mini Kit provides the following advantages: • Rapid and efficient purification of high-quality viral nucleic acid using spin column-based centrifugation with no sample cross-contamination • Specifically designed to purify viral RNA and DNA from ≤500 μL cell-free samples within 45 min • Ability to elute viral nucleic acids in low elution volumes of 10–50 μL to allow sensitive downstream analysis Order Info Shipping Condition: Room Temperature
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Column Type: Spin Column
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: Viral RNA and DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: 5 μg (Binding capacity)
Content And Storage: • 310 μg Carrier RNA; –20°C• 32 mL Viral Lysis Buffer L22; room temperature• 15 mL Wash Buffer WII; room temperature• 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage• 15.5 mL RNase-free Water E3; room temperature• 50 Viral Spin Columns with Collection Tubes; room temperature• 2 x 50 Wash Tubes; room temperature• 50 Recovery Tubes; room temperature
Sample Type: Cells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM)
Elution Volume: 10 to 50 μL
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity: 50 Preps
Test Time: 45 min.
Starting Material Amount: ≤500 μL
Column Type:
Spin Column
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
Viral RNA and DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
5 μg (Binding capacity)
Content And Storage:
• 310 μg Carrier RNA; –20°C• 32 mL Viral Lysis Buffer L22; room temperature• 15 mL Wash Buffer WII; room temperature• 2 x 1.6 mL Proteinase K; room temperature, or 4°C for long-term storage• 15.5 mL RNase-free Water E3; room temperature• 50 Viral Spin Columns with Collection Tubes; room temperature• 2 x 50 Wash Tubes; room temperature• 50 Recovery Tubes; room temperature
Sample Type:
Cells, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM)
Elution Volume:
10 to 50 μL
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity:
50 Preps
Test Time:
45 min.
Starting Material Amount:
≤500 μL
Column Type: Spin Column
Isolation Technology: Silica Plate
Scale: Mini
Final Product Type: Viral RNA and DNA
High-throughput Compatibility: High-throughput Compatible
Shipping Condition: Room Temperature
Yield: >40 μg (Binding capacity)
Content And Storage: • 2.2 mg Carrier RNA; –20°C• 100 mL PureLink Pro 96 Viral Lysis Buffer L22; room temperature• 2 x 87.5 mL PureLink Pro 96 Wash Buffer II; room temperature• 10 mL Proteinase K; room temperature, or 4°C for long-term storage• 75 mL RNase-free Water; room temperature• 4 PureLink 96 Well Filter Plates; room temperature• 2 x 6/bag 96 Deep-Well Block; room temperature• 3 x 4/bag Foil Tape; room temperature
Sample Type: Cells, Cerebrospinal Fluid, Plasma, Serum
Elution Volume: 100 to 150 μL
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity: 4 plates
Test Time: 30 min.
Starting Material Amount: 200 μL
Column Type:
Spin Column
Isolation Technology:
Silica Plate
Scale:
Mini
Final Product Type:
Viral RNA and DNA
High-throughput Compatibility:
High-throughput Compatible
Shipping Condition:
Room Temperature
Yield:
>40 μg (Binding capacity)
Content And Storage:
• 2.2 mg Carrier RNA; –20°C• 100 mL PureLink Pro 96 Viral Lysis Buffer L22; room temperature• 2 x 87.5 mL PureLink Pro 96 Wash Buffer II; room temperature• 10 mL Proteinase K; room temperature, or 4°C for long-term storage• 75 mL RNase-free Water; room temperature• 4 PureLink 96 Well Filter Plates; room temperature• 2 x 6/bag 96 Deep-Well Block; room temperature• 3 x 4/bag Foil Tape; room temperature
Sample Type:
Cells, Cerebrospinal Fluid, Plasma, Serum
Elution Volume:
100 to 150 μL
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity:
4 plates
Test Time:
30 min.
Starting Material Amount:
200 μL
Column Type: __
Isolation Technology: __
Scale: __
Final Product Type: __
High-throughput Compatibility: __
Shipping Condition: __
Yield: __
Content And Storage: All destination vectors are provided lyophilized and supercoiled.
Sample Type: __
Elution Volume: __
For Use With (Application): Constitutive Expression
Quantity: 6 μg
Test Time: __
Starting Material Amount: __
Column Type:
__
Isolation Technology:
__
Scale:
__
Final Product Type:
__
High-throughput Compatibility:
__
Shipping Condition:
__
Yield:
__
Content And Storage:
All destination vectors are provided lyophilized and supercoiled.
Sample Type:
__
Elution Volume:
__
For Use With (Application):
Constitutive Expression
Quantity:
6 μg
Test Time:
__
Starting Material Amount:
__
Column Type: Spin Column
Isolation Technology: __
Scale: __
Final Product Type: __
High-throughput Compatibility: __
Shipping Condition: __
Yield: __
Content And Storage: Store at room temperature.
Sample Type: Liquid Samples (e.g. Serum), Virus
Elution Volume: __
For Use With (Application): Nucleic Acid Extraction
Quantity: 100 Tubes
Test Time: __
Starting Material Amount: __
Column Type:
Spin Column
Isolation Technology:
__
Scale:
__
Final Product Type:
__
High-throughput Compatibility:
__
Shipping Condition:
__
Yield:
__
Content And Storage:
Store at room temperature.
Sample Type:
Liquid Samples (e.g. Serum), Virus
Elution Volume:
__
For Use With (Application):
Nucleic Acid Extraction
Quantity:
100 Tubes
Test Time:
__
Starting Material Amount:
__