A42358
Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes
Manufacturer: Applied Biosystems™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A42358-Each-of-1 | In Stock | ₹ 61,260.96 |
A42358 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 61,260.96
GST (18%): ₹ 11,026.973
Total Price: ₹ 72,287.933
Content And Storage
• 80 mL Lysis Buffer; room temperature• 50 mL Binding Solution; room temperature• 200 mL Wash Buffer; room temperature• 20 mL Elution Solution; room temperature• 4 mL Proteinase K; room temperature• 2 mL Total Nucleic Acid Binding Beads; room temperature• 100 Tubes; room temperature
Sample Type
Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
Final Product Type
RNA and DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Starting Material Amount
Bacteria: ≤500 μLBiofluids: ≤500 μLFecal: ≤100 mg (200 μL for samples in storage solution)Soil: ≤250 mgYeast: ≤500 μL
Isolation Technology
Magnetic Bead
Elution Volume
50 to 200 μL
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity
100 preps
Test Time
60 min.
Related Products
Description
- Stool and soil are often the sample types of choice for scientists in the microbiome field
- However, these samples also happen to be some of the most challenging to extract RNA and DNA from, until now
- The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from stool and soil
- The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing
- Features of the MagMAX Microbiome Nucleic Acid Isolation Kit include: • Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems • Fast procedure allows for 96 samples to be processed in ∼60 minutes • Compatible with stool, soil, and swabs contained in transport medium • Mechanical disruption via bead beating in 96-well plate or individual tubes • Elution volumes ranging from 50 to 200 μL Maximize sample input and nucleic acid recovery in minimal time Up to 100 mg of stool can be processed per well in a KingFisher 96-deep well plate
- When the extraction process is performed using a KingFisher Flex system, 96 samples can processed in ∼60 minutes; when using a KingFisher Duo Prime system 12 samples can be processed in ∼50 minutes
- When the protocol is completed, pure RNA and DNA is concentrated in 100 μL of elution buffer
- Two bead beating formats available High-throughput processing of difficult samples such as stool is possible with 96-deep well plates containing zirconia beads combined with optimized MagMAX Microbiome reagents
- If processing 96 samples at a time is not needed then the same beads are available in tube format for lower-throughput processing
- Once the bead beating step is complete, the lysate can then be transferred into a KingFisher 96-deep well plate and processed on a KingFisher Flex or KingFisher Duo Prime system
- Benefits of MagMAX magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid
- Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields
- Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples
- This clogging issue is of particular concern with stool samples that are commonly used for microbiome research
- The Dynabeads MyOne Silane contained in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture
- These aspects make Dynabeads MyOne Silane ideal for the recovery RNA and DNA
- Once captured, these nucleic acids can then be eluted in of 50-100 μL of elution buffer for use in downstream applications.
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Content And Storage: • 80 mL Lysis Buffer; room temperature• 50 mL Binding Solution; room temperature• 200 mL Wash Buffer; room temperature• 20 mL Elution Solution; room temperature• 4 mL Proteinase K; room temperature• 2 mL Total Nucleic Acid Binding Beads; room temperature• 100 Tubes; room temperature
Sample Type: Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
Final Product Type: RNA and DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤500 μLBiofluids: ≤500 μLFecal: ≤100 mg (200 μL for samples in storage solution)Soil: ≤250 mgYeast: ≤500 μL
Isolation Technology: Magnetic Bead
Elution Volume: 50 to 200 μL
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity: 100 preps
Test Time: 60 min.
Content And Storage:
• 80 mL Lysis Buffer; room temperature• 50 mL Binding Solution; room temperature• 200 mL Wash Buffer; room temperature• 20 mL Elution Solution; room temperature• 4 mL Proteinase K; room temperature• 2 mL Total Nucleic Acid Binding Beads; room temperature• 100 Tubes; room temperature
Sample Type:
Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
Final Product Type:
RNA and DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Starting Material Amount:
Bacteria: ≤500 μLBiofluids: ≤500 μLFecal: ≤100 mg (200 μL for samples in storage solution)Soil: ≤250 mgYeast: ≤500 μL
Isolation Technology:
Magnetic Bead
Elution Volume:
50 to 200 μL
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity:
100 preps
Test Time:
60 min.
Content And Storage: Store at room temperature.
Sample Type: Bacteria, Blood, Buccal Samples, Cells, Fungi, Liquid Samples (e.g. Serum), Virus, Yeast
Final Product Type: __
High-throughput Compatibility: High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition: Room Temperature
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
For Use With (Application): Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity: 550 mL
Test Time: __
Content And Storage:
Store at room temperature.
Sample Type:
Bacteria, Blood, Buccal Samples, Cells, Fungi, Liquid Samples (e.g. Serum), Virus, Yeast
Final Product Type:
__
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition:
Room Temperature
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
__
For Use With (Application):
Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity:
550 mL
Test Time:
__
Content And Storage: Store at room temperature.
Sample Type: Serum, Viral Samples, Buccal Samples, Blood, Cells, Bacteria, Fungi, Yeast
Final Product Type: __
High-throughput Compatibility: High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition: Room Temperature
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
For Use With (Application): Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity: 1000 mL
Test Time: __
Content And Storage:
Store at room temperature.
Sample Type:
Serum, Viral Samples, Buccal Samples, Blood, Cells, Bacteria, Fungi, Yeast
Final Product Type:
__
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition:
Room Temperature
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
__
For Use With (Application):
Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity:
1000 mL
Test Time:
__
Content And Storage: Store at room temperature.
Sample Type: Bacteria, Blood, Buccal Samples, Cells, Fungi, Liquid Samples (e.g. Serum), Viral Samples, Yeast
Final Product Type: __
High-throughput Compatibility: High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition: Room Temperature
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
For Use With (Application): Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity: 800 mL
Test Time: __
Content And Storage:
Store at room temperature.
Sample Type:
Bacteria, Blood, Buccal Samples, Cells, Fungi, Liquid Samples (e.g. Serum), Viral Samples, Yeast
Final Product Type:
__
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition:
Room Temperature
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
__
For Use With (Application):
Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity:
800 mL
Test Time:
__