A42357
Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead plate
Manufacturer: Applied Biosystems™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A42357-Each-of-1 | In Stock | ₹ 64,614.00 |
A42357 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 64,614.00
GST (18%): ₹ 11,630.52
Total Price: ₹ 76,244.52
Content And Storage
• 80 mL Lysis Buffer; room temperature• 50 mL Binding Solution; room temperature• 200 mL Wash Buffer; room temperature• 20 mL Elution Solution; room temperature• 4 mL Proteinase K; room temperature• 2 mL Total Nucleic Acid Binding Beads; room temperature• 1 96-Deep Well Plate; room temperature
Sample Type
Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
Final Product Type
RNA and DNA
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity
100 Preps
Test Time
Up to 35 min. when using automated protocol
Isolation Technology
Magnetic Bead
Elution Volume
50 to 200 μL
For Use With (Equipment)
KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
High-throughput Compatibility
High-throughput Compatible
Shipping Condition
Room Temperature
Starting Material Amount
Bacteria: ≤500 μLBiofluids: ≤500 μLFecal: ≤100 mg (200 μL for samples in storage solution)Soil: ≤250 mgYeast: ≤500 μL
Related Products
Description
- Stool and soil are often the sample types of choice for scientists in the microbiome field
- However, these samples also happen to be some of the most challenging to extract RNA and DNA from, until now
- The MagMAX Microbiome Ultra Nucleic Acid Isolation Kit enables efficient, high-throughput nucleic acid extraction from stool and soil
- The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing
- Features of the MagMAX Microbiome Nucleic Acid Isolation Kit include: • Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems • Fast procedure allows for 96 samples to be processed in ∼60 minutes • Compatible with stool, soil, and swabs contained in transport medium • Mechanical disruption via bead beating in 96-well plate or individual tubes • Elution volumes ranging from 50 to 200 μL Maximize sample input and nucleic acid recovery in minimal time Up to 100 mg of stool can be processed per well in a KingFisher 96-deep well plate
- When the extraction process is performed using a KingFisher Flex system, 96 samples can processed in ∼60 minutes; when using a KingFisher Duo Prime system 12 samples can be processed in ∼50 minutes
- When the protocol is completed, pure RNA and DNA is concentrated in 100 μL of elution buffer
- Two bead beating formats available High-throughput processing of difficult samples such as stool is possible with 96-deep well plates containing zirconia beads combined with optimized MagMAX Microbiome reagents
- If processing 96 samples at a time is not needed then the same beads are available in tube format for lower-throughput processing
- Once the bead beating step is complete, the lysate can then be transferred into a KingFisher 96-deep well plate and processed on a KingFisher Flex or KingFisher Duo Prime system
- Benefits of MagMAX magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid
- Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields
- Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples
- This clogging issue is of particular concern with stool samples that are commonly used for microbiome research
- The Dynabeads MyOne Silane contained in the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture
- These aspects make Dynabeads MyOne Silane ideal for the recovery RNA and DNA
- Once captured, these nucleic acids can then be eluted in of 50-100 μL of elution buffer for use in downstream applications.
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Content And Storage: • 80 mL Lysis Buffer; room temperature• 50 mL Binding Solution; room temperature• 200 mL Wash Buffer; room temperature• 20 mL Elution Solution; room temperature• 4 mL Proteinase K; room temperature• 2 mL Total Nucleic Acid Binding Beads; room temperature• 1 96-Deep Well Plate; room temperature
Sample Type: Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
Final Product Type: RNA and DNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity: 100 Preps
Test Time: Up to 35 min. when using automated protocol
Isolation Technology: Magnetic Bead
Elution Volume: 50 to 200 μL
For Use With (Equipment): KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
High-throughput Compatibility: High-throughput Compatible
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤500 μLBiofluids: ≤500 μLFecal: ≤100 mg (200 μL for samples in storage solution)Soil: ≤250 mgYeast: ≤500 μL
Content And Storage:
• 80 mL Lysis Buffer; room temperature• 50 mL Binding Solution; room temperature• 200 mL Wash Buffer; room temperature• 20 mL Elution Solution; room temperature• 4 mL Proteinase K; room temperature• 2 mL Total Nucleic Acid Binding Beads; room temperature• 1 96-Deep Well Plate; room temperature
Sample Type:
Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
Final Product Type:
RNA and DNA
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity:
100 Preps
Test Time:
Up to 35 min. when using automated protocol
Isolation Technology:
Magnetic Bead
Elution Volume:
50 to 200 μL
For Use With (Equipment):
KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
High-throughput Compatibility:
High-throughput Compatible
Shipping Condition:
Room Temperature
Starting Material Amount:
Bacteria: ≤500 μLBiofluids: ≤500 μLFecal: ≤100 mg (200 μL for samples in storage solution)Soil: ≤250 mgYeast: ≤500 μL
Content And Storage: • 80 mL Lysis Buffer; room temperature• 50 mL Binding Solution; room temperature• 200 mL Wash Buffer; room temperature• 20 mL Elution Solution; room temperature• 4 mL Proteinase K; room temperature• 2 mL Total Nucleic Acid Binding Beads; room temperature• 100 Tubes; room temperature
Sample Type: Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
Final Product Type: RNA and DNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity: 100 preps
Test Time: 60 min.
Isolation Technology: Magnetic Bead
Elution Volume: 50 to 200 μL
For Use With (Equipment): __
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤500 μLBiofluids: ≤500 μLFecal: ≤100 mg (200 μL for samples in storage solution)Soil: ≤250 mgYeast: ≤500 μL
Content And Storage:
• 80 mL Lysis Buffer; room temperature• 50 mL Binding Solution; room temperature• 200 mL Wash Buffer; room temperature• 20 mL Elution Solution; room temperature• 4 mL Proteinase K; room temperature• 2 mL Total Nucleic Acid Binding Beads; room temperature• 100 Tubes; room temperature
Sample Type:
Bacteria, Biofluids, Fecal, Soil, Swabs (Buccal or Skin), Yeast
Final Product Type:
RNA and DNA
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity:
100 preps
Test Time:
60 min.
Isolation Technology:
Magnetic Bead
Elution Volume:
50 to 200 μL
For Use With (Equipment):
__
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Starting Material Amount:
Bacteria: ≤500 μLBiofluids: ≤500 μLFecal: ≤100 mg (200 μL for samples in storage solution)Soil: ≤250 mgYeast: ≤500 μL
Content And Storage: Store at room temperature.
Sample Type: Bacteria, Blood, Buccal Samples, Cells, Fungi, Liquid Samples (e.g. Serum), Virus, Yeast
Final Product Type: __
For Use With (Application): Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity: 550 mL
Test Time: __
Isolation Technology: __
Elution Volume: __
For Use With (Equipment): __
High-throughput Compatibility: High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition: Room Temperature
Starting Material Amount: __
Content And Storage:
Store at room temperature.
Sample Type:
Bacteria, Blood, Buccal Samples, Cells, Fungi, Liquid Samples (e.g. Serum), Virus, Yeast
Final Product Type:
__
For Use With (Application):
Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity:
550 mL
Test Time:
__
Isolation Technology:
__
Elution Volume:
__
For Use With (Equipment):
__
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition:
Room Temperature
Starting Material Amount:
__
Content And Storage: Store at room temperature.
Sample Type: Serum, Viral Samples, Buccal Samples, Blood, Cells, Bacteria, Fungi, Yeast
Final Product Type: __
For Use With (Application): Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity: 1000 mL
Test Time: __
Isolation Technology: __
Elution Volume: __
For Use With (Equipment): __
High-throughput Compatibility: High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition: Room Temperature
Starting Material Amount: __
Content And Storage:
Store at room temperature.
Sample Type:
Serum, Viral Samples, Buccal Samples, Blood, Cells, Bacteria, Fungi, Yeast
Final Product Type:
__
For Use With (Application):
Next-Generation Sequencing, RT-PCR (Endpoint), Real-Time PCR
Quantity:
1000 mL
Test Time:
__
Isolation Technology:
__
Elution Volume:
__
For Use With (Equipment):
__
High-throughput Compatibility:
High-throughput Compatible, Manual Protocols, Automated Protocols
Shipping Condition:
Room Temperature
Starting Material Amount:
__