Cytiva TempliPhi™ 100/500 Amplification Kit
Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
Manufacturer: Fischer Scientific
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Content And Storage
The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification
For Use With (Application)
Sequencing Template Amplification Kits and Reagents
Test Time
4-18 h depending on starting material
Description
illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
Includes
0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA
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Description
- Description Generate microgram quantities of template DNA from picogram amounts of starting material Completed reactions contain uniform quantities of DNA irrespective of input amount, allowing for use directly in sequencing reactions without quantification Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA without the need for purification Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation Contain separate, unmixed components for enhanced stability
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Content And Storage: The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification
For Use With (Application): Sequencing Template Amplification Kits and Reagents
Test Time: 4-18 h depending on starting material
Description: illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
Includes: 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA
Content And Storage: Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
For Use With (Application): Amplification of difficult templates for successful DNA sequencing
Test Time: 18 h
Description: illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
Includes: __
Content And Storage: Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
For Use With (Application): Amplification of difficult templates for successful DNA sequencing
Test Time: 18 h
Description: illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
Includes: __
