45-001-494
Cytiva bacteria genomicPrep Mini Spin Kit
Manufacturer: Cytiva
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 45-001-494-Each-of-1 | In Stock | ₹ 1,41,858.48 |
45-001-494 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,41,858.48
GST (18%): ₹ 25,534.526
Total Price: ₹ 1,67,393.006
Description
illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria.
Includes
30mg Proteinase K, 3mL Lysis Buffer type 2, 0.6mL Lysis Buffer type 3, 60mL Lysis Buffer type 4, 6mL Wash Buffer type 6, 12mL Elution Buffer type 5, 50 Illustra™ bacteria mini columns, 50 collection tubes
Product Type
illustra bacteria genomicPrep Mini Spin Kit
Sample Volume
2 x 109cells [A600= 2.0]
For Use With (Application)
For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping
No. of Reactions
250 Preps
Purity
1.7
Test Time
40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria]
Related Products
Description
- The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc
- The kit produces highly pure and intact genomic DNA with OD 260/280 values of 1.75-1.85
- Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR
- The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells
- The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution
- Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution
- Total DNA extraction time under one hour with reduced lysis time Can be used to purify 4 to 8μg of genomic DNA from 2 × 10 9 bacteria cells with input amounts ranging from 1 × 10 9 to 4 × 10 9 bacteria cells Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency Validated in several downstream applications including RT PCR, long PCR, and restriction digest Color-coded caps for ease of use
Compare Similar Items
Show Difference
Description: illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria.
Includes: 30mg Proteinase K, 3mL Lysis Buffer type 2, 0.6mL Lysis Buffer type 3, 60mL Lysis Buffer type 4, 6mL Wash Buffer type 6, 12mL Elution Buffer type 5, 50 Illustra™ bacteria mini columns, 50 collection tubes
Product Type: illustra bacteria genomicPrep Mini Spin Kit
Sample Volume: 2 x 109cells [A600= 2.0]
For Use With (Application): For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping
No. of Reactions: 250 Preps
Purity: 1.7
Test Time: 40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria]
Description:
illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria.
Includes:
30mg Proteinase K, 3mL Lysis Buffer type 2, 0.6mL Lysis Buffer type 3, 60mL Lysis Buffer type 4, 6mL Wash Buffer type 6, 12mL Elution Buffer type 5, 50 Illustra™ bacteria mini columns, 50 collection tubes
Product Type:
illustra bacteria genomicPrep Mini Spin Kit
Sample Volume:
2 x 109cells [A600= 2.0]
For Use With (Application):
For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping
No. of Reactions:
250 Preps
Purity:
1.7
Test Time:
40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria]
Description: illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
Includes: Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
Product Type: illustra blood genomicPrep Mini Spin Kit
Sample Volume: 50 μl whole blood, Buffy coat, bone marrow cells, 2 μl nucleated blood†, 300 μl whole blood‡
For Use With (Application): Used for several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digestsSuitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; rest
No. of Reactions: 50 Preps
Purity: 1.7
Test Time: < 20 min
Description:
illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
Includes:
Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
Product Type:
illustra blood genomicPrep Mini Spin Kit
Sample Volume:
50 μl whole blood, Buffy coat, bone marrow cells, 2 μl nucleated blood†, 300 μl whole blood‡
For Use With (Application):
Used for several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digestsSuitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; rest
No. of Reactions:
50 Preps
Purity:
1.7
Test Time:
< 20 min
Description: illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
Includes: Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
Product Type: illustra blood genomicPrep Mini Spin Kit
Sample Volume: 50 μl whole blood, Buffy coat, bone marrow cells, 2 μl nucleated blood†, 300 μl whole blood‡
For Use With (Application): Used for several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digestsSuitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; rest
No. of Reactions: 250 Preps
Purity: 1.7
Test Time: < 20 min
Description:
illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
Includes:
Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
Product Type:
illustra blood genomicPrep Mini Spin Kit
Sample Volume:
50 μl whole blood, Buffy coat, bone marrow cells, 2 μl nucleated blood†, 300 μl whole blood‡
For Use With (Application):
Used for several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digestsSuitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; rest
No. of Reactions:
250 Preps
Purity:
1.7
Test Time:
< 20 min
Description: illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume.
Includes: Fast-flow genomic 250 columns, lyophilized Proteinase K powder, Lysis solutions, load and Elution Buffers, NAP-25 desalting column, spin column adapters, full protocol booklet, detachable, quick reference protocol card
Product Type: illustra plasmidPrep Midi Flow Kit
Sample Volume: 150 mL Culture
For Use With (Application): Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification
No. of Reactions: 25 Preps
Purity: 1.8
Test Time: Approx.215 min
Description:
illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume.
Includes:
Fast-flow genomic 250 columns, lyophilized Proteinase K powder, Lysis solutions, load and Elution Buffers, NAP-25 desalting column, spin column adapters, full protocol booklet, detachable, quick reference protocol card
Product Type:
illustra plasmidPrep Midi Flow Kit
Sample Volume:
150 mL Culture
For Use With (Application):
Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification
No. of Reactions:
25 Preps
Purity:
1.8
Test Time:
Approx.215 min