Cytiva bacteria genomicPrep Mini Spin Kit
Manufacturer: Cytiva
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 45-001-494-Each-of-1 | In Stock | ₹ 1,47,562.00 |
45-001-494 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,47,562.00
GST (18%): ₹ 26,561.16
Total Price: ₹ 1,74,123.16
Description
illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria.
Includes
30mg Proteinase K, 3mL Lysis Buffer type 2, 0.6mL Lysis Buffer type 3, 60mL Lysis Buffer type 4, 6mL Wash Buffer type 6, 12mL Elution Buffer type 5, 50 Illustra™ bacteria mini columns, 50 collection tubes
Product Type
illustra bacteria genomicPrep Mini Spin Kit
Sample Volume
2 x 109cells [A600= 2.0]
For Use With (Application)
For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping
No. of Reactions
250 Preps
Purity
1.7
Test Time
40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria]
Related Products
Description
- The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc
- The kit produces highly pure and intact genomic DNA with OD 260/280 values of 1.75-1.85
- Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR
- The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells
- The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution
- Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution
- Total DNA extraction time under one hour with reduced lysis time Can be used to purify 4 to 8μg of genomic DNA from 2 × 10 9 bacteria cells with input amounts ranging from 1 × 10 9 to 4 × 10 9 bacteria cells Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency Validated in several downstream applications including RT PCR, long PCR, and restriction digest Color-coded caps for ease of use