Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit
The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.
Manufacturer: Fischer Scientific
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Content And Storage
• Resuspension buffer, store at 4°C upon reciept• Lysis buffer, store at room temperature• Precipitation buffer, store at room temperature• Binding buffer, store at room temperature• Wash buffer 1, store at room temperature• Wash buffer 2 (concentrate), store at room temperature• Elution buffer, store at room temperature• Column assemblies, store at room temperature• Syringe filters, store at room temperature• Syringe plungers, store at room temperature• Collection tubes, store at room temperature
Isolation Technology
Silica Column
For Use With (Application)
Cloning, PCR
Product Line
PureLink™
Shipping Condition
Room Temperature
Format
Kit
Scale
Midi
Prep Scale
Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg
Product Type
Plasmid Purification Kit
Test Time
30 min.
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Description
- The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing
- Ultra-fast protocol —innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes Low endotoxin plasmid —the resulting plasmid contains less than 1 EU/μg endotoxins High yields —isolate up to 0.4 mg of high-quality plasmid DNA Colored buffers —the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
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Content And Storage: • Resuspension buffer, store at 4°C upon reciept• Lysis buffer, store at room temperature• Precipitation buffer, store at room temperature• Binding buffer, store at room temperature• Wash buffer 1, store at room temperature• Wash buffer 2 (concentrate), store at room temperature• Elution buffer, store at room temperature• Column assemblies, store at room temperature• Syringe filters, store at room temperature• Syringe plungers, store at room temperature• Collection tubes, store at room temperature
Isolation Technology: Silica Column
For Use With (Application): Cloning, PCR
Product Line: PureLink™
Shipping Condition: Room Temperature
Format: Kit
Scale: Midi
Prep Scale: Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg
Product Type: Plasmid Purification Kit
Test Time: 30 min.
Content And Storage: • Resuspension buffer, store at 4°C upon reciept• Lysis buffer, store at room temperature• Precipitation buffer, store at room temperature• Binding buffer, store at room temperature• Wash buffer 1, store at room temperature• Wash buffer 2 (concentrate), store at room temperature• Elution buffer, store at room temperature• Column assemblies, store at room temperature• Syringe filters, store at room temperature• Syringe plungers, store at room temperature• Collection tubes, store at room temperature
Isolation Technology: Silica Column
For Use With (Application): Cloning, PCR
Product Line: PureLink™
Shipping Condition: Room Temperature
Format: Kit
Scale: Maxi
Prep Scale: Maxi input culture of 100-500 mL to obtain a yield of up to 850 μg
Product Type: Plasmid Purification Kit
Test Time: 30 min.
Content And Storage: • Resuspension buffer, store at 4°C upon reciept• Lysis buffer, store at room temperature• Precipitation buffer, store at room temperature• Binding buffer, store at room temperature• Wash buffer 1, store at room temperature• Wash buffer 2 (concentrate), store at room temperature• Elution buffer, store at room temperature• Column assemblies, store at room temperature• Syringe filters, store at room temperature• Syringe plungers, store at room temperature• Collection tubes, store at room temperature
Isolation Technology: Silica Column
For Use With (Application): Cloning, PCR
Product Line: PureLink™
Shipping Condition: Room Temperature
Format: Kit
Scale: Maxi
Prep Scale: Maxi input culture of 100-500 mL to obtain a yield of up to 850 μg
Product Type: Plasmid Purification Kit
Test Time: 30 min.
