FERK0792
Thermo Scientific™ GeneJET Plant Genomic DNA Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERK0792-Each-of-1 | In Stock | ₹ 83,207.10 |
FERK0792 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 83,207.10
GST (18%): ₹ 14,977.278
Total Price: ₹ 98,184.378
Column Type
Spin Column
Isolation Technology
Silica Spin Column
Scale
Mini
Final Product Type
gDNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Yield
≤32 μg per 100 mg tissue
Content And Storage
• 6 x 1 mL RNase A; store at room temperature until opened, –20°C after opening• 120 mL Lysis Buffer A; room temperature• 15 mL Lysis Buffer B; room temperature• 40 mL Precipitation Solution; room temperature• 120 mL Plant gDNA Binding Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Sample Type
Plants
Elution Volume
200 μL
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity
250 Preps
Test Time
30 min. (After sample homogenization)
Starting Material Amount
≤100 mg of fresh/frozen≤20 mg lyophilized
Related Products
Description
- GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types
- It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
- Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types
- It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
- Highlights • Fast —procedure completed in 30 min following cell lysis • Convenient —protocol does not include clogging-prone lysate filtration step • High yield —up to 32 μg of high quality DNA from 100 mg sample, including nuclear, mitochondrial, and chloroplast DNA • Compatible —use with awide variety of samples (leaves, roots, sprouts and other parts of various plants) The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size
- DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample
- The typical yield from a standard tissue source (e
- g
- young wheat leaves) is 30 to 32 μg from 100 mg of tissue
- Isolated DNA can be used directly in PCR, qPCR, southern blotting, and enzymatic reactions
- Applications • Isolated genomic DNA is ideal for use in common molecular biology procedures, including: • PCR and qPCR • FastDigest and conventional restriction digestion • Southern blotting
Compare Similar Items
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Column Type: Spin Column
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: gDNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: ≤32 μg per 100 mg tissue
Content And Storage: • 6 x 1 mL RNase A; store at room temperature until opened, –20°C after opening• 120 mL Lysis Buffer A; room temperature• 15 mL Lysis Buffer B; room temperature• 40 mL Precipitation Solution; room temperature• 120 mL Plant gDNA Binding Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Sample Type: Plants
Elution Volume: 200 μL
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity: 250 Preps
Test Time: 30 min. (After sample homogenization)
Starting Material Amount: ≤100 mg of fresh/frozen≤20 mg lyophilized
Column Type:
Spin Column
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
gDNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
≤32 μg per 100 mg tissue
Content And Storage:
• 6 x 1 mL RNase A; store at room temperature until opened, –20°C after opening• 120 mL Lysis Buffer A; room temperature• 15 mL Lysis Buffer B; room temperature• 40 mL Precipitation Solution; room temperature• 120 mL Plant gDNA Binding Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Sample Type:
Plants
Elution Volume:
200 μL
For Use With (Application):
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity:
250 Preps
Test Time:
30 min. (After sample homogenization)
Starting Material Amount:
≤100 mg of fresh/frozen≤20 mg lyophilized
Column Type: __
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: Total RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: Up to 65 μg
Content And Storage: • 40 mL Plant RNA Lysis Solution; store at room temperature• 40 mL Wash Buffer WB 1 (concentrated); room temperature• 30 mL Wash Buffer 2 (concentrated); room temperature• 30 mL Water, Nuclease-free; room temperature• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 50 Collection Tubes (2 mL); room temperature• 50 Collection Tubes (1.5 mL); room temperature
Sample Type: Plants
Elution Volume: 50 μL
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity: 50 Preps
Test Time: __
Starting Material Amount: ≤100 mg
Column Type:
__
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
Total RNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
Up to 65 μg
Content And Storage:
• 40 mL Plant RNA Lysis Solution; store at room temperature• 40 mL Wash Buffer WB 1 (concentrated); room temperature• 30 mL Wash Buffer 2 (concentrated); room temperature• 30 mL Water, Nuclease-free; room temperature• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 50 Collection Tubes (2 mL); room temperature• 50 Collection Tubes (1.5 mL); room temperature
Sample Type:
Plants
Elution Volume:
50 μL
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity:
50 Preps
Test Time:
__
Starting Material Amount:
≤100 mg
Column Type: __
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: Total RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: Up to 65 μg
Content And Storage: • 200 mL Plant RNA Lysis Solution; store at room temperature• 200 mL Wash Buffer WB 1 (concentrated); room temperature• 2 x 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Water, Nuclease-free; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Sample Type: Plants
Elution Volume: 50 μL
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity: 250 Preps
Test Time: __
Starting Material Amount: ≤100 mg
Column Type:
__
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
Total RNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
Up to 65 μg
Content And Storage:
• 200 mL Plant RNA Lysis Solution; store at room temperature• 200 mL Wash Buffer WB 1 (concentrated); room temperature• 2 x 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Water, Nuclease-free; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Sample Type:
Plants
Elution Volume:
50 μL
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity:
250 Preps
Test Time:
__
Starting Material Amount:
≤100 mg
Column Type: Spin Column
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: Viral DNA and RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: 50 μg (Binding capacity)
Content And Storage: • 2 x 1.4 mL Column Preparation Liquid; room temperature• 12 mL Lysis Solution; room temperature• 25 mL Wash Buffer 1 (concentrated); room temperature• 11 mL Wash Buffer 2 (concentrated); room temperature• 3 x 1.25 mL Eluent; room temperature• 50 Spin Columns with Wash Tubes; room temperature• 4 x 50 Wash Tubes; room temperature• 2 x 1.3 mL Proteinase K; –20°C• 1 vial Carrier RNA; –20°C
Sample Type: Milk, Plasma, Saliva, Serum, Swabs (Nasal, Buccal, or Urogenital), Urine, Whole Blood, Virus
Elution Volume: 50 μL
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity: 50 Preps
Test Time: 30 min.
Starting Material Amount: 200 μL
Column Type:
Spin Column
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
Viral DNA and RNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
50 μg (Binding capacity)
Content And Storage:
• 2 x 1.4 mL Column Preparation Liquid; room temperature• 12 mL Lysis Solution; room temperature• 25 mL Wash Buffer 1 (concentrated); room temperature• 11 mL Wash Buffer 2 (concentrated); room temperature• 3 x 1.25 mL Eluent; room temperature• 50 Spin Columns with Wash Tubes; room temperature• 4 x 50 Wash Tubes; room temperature• 2 x 1.3 mL Proteinase K; –20°C• 1 vial Carrier RNA; –20°C
Sample Type:
Milk, Plasma, Saliva, Serum, Swabs (Nasal, Buccal, or Urogenital), Urine, Whole Blood, Virus
Elution Volume:
50 μL
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
Quantity:
50 Preps
Test Time:
30 min.
Starting Material Amount:
200 μL