FERK0722
Thermo Scientific™ GeneJET Genomic DNA Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERK0722-Each-of-1 | In Stock | ₹ 69,132.48 |
FERK0722 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 69,132.48
GST (18%): ₹ 12,443.846
Total Price: ₹ 81,576.326
Column Type
Spin Column
Isolation Technology
Silica Spin Column
Scale
Mini
Final Product Type
gDNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Yield
≤30 μg
Content And Storage
• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening• 55 mL Digestion Solution; room temperature• 2 x 60 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Sample Type
Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
Elution Volume
200 μL
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity
250 Preps
Test Time
20 min. (After sample homogenization)
Starting Material Amount
Bacteria: ≤109cellsCells: ≤106Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)Tissue: ≤20 mgWhole Blood: ≤200 μLYeast: ≤108cells
Related Products
Description
- Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast
- The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
- The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size
- Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions
- Highlights • Universal —can be used for both cell and tissue samples from a wide range of sources • Efficient —high yields of high molecular weight genomic DNA • Fast —20 minute procedure following cell lysis • Convenient —spin columns are capped and assembled with collection tubes Applications Isolated genomic DNA is ideal for use in common molecular biology procedures, including: • PCR and qPCR • FastDigest and conventional restriction digestion • Southern blotting
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Column Type: Spin Column
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: gDNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: ≤30 μg
Content And Storage: • 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening• 55 mL Digestion Solution; room temperature• 2 x 60 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Sample Type: Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
Elution Volume: 200 μL
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity: 250 Preps
Test Time: 20 min. (After sample homogenization)
Starting Material Amount: Bacteria: ≤109cellsCells: ≤106Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)Tissue: ≤20 mgWhole Blood: ≤200 μLYeast: ≤108cells
Column Type:
Spin Column
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
gDNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
≤30 μg
Content And Storage:
• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening• 55 mL Digestion Solution; room temperature• 2 x 60 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Sample Type:
Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
Elution Volume:
200 μL
For Use With (Application):
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity:
250 Preps
Test Time:
20 min. (After sample homogenization)
Starting Material Amount:
Bacteria: ≤109cellsCells: ≤106Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)Tissue: ≤20 mgWhole Blood: ≤200 μLYeast: ≤108cells
Column Type: __
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: Total RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: Up to 160 μg
Content And Storage: • 600 μL Proteinase K; store at room temperature until opened, -20°C after opening• 40 mL Lysis Buffer; room temperature• 40 mL Wash Buffer 1 (concentrated); room temperature• 23 mL Wash Buffer 2 (concentrated); room temperature• 30 mL Nuclease-free Water; room temperature• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 50 Collection Tubes (2 mL); room temperature• 50 Collection Tubes (1.5 mL); room temperature
Sample Type: Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Elution Volume: 50–100 μL
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity: 50 Preps
Test Time: __
Starting Material Amount: Bacteria: ≤109cellsBlood: 0.5 mLCells: ≤107Tissue and Insects: 30 mgYeast: ≤4 x 108cellsEnzymatic reactions: ≤100 μL
Column Type:
__
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
Total RNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
Up to 160 μg
Content And Storage:
• 600 μL Proteinase K; store at room temperature until opened, -20°C after opening• 40 mL Lysis Buffer; room temperature• 40 mL Wash Buffer 1 (concentrated); room temperature• 23 mL Wash Buffer 2 (concentrated); room temperature• 30 mL Nuclease-free Water; room temperature• 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 50 Collection Tubes (2 mL); room temperature• 50 Collection Tubes (1.5 mL); room temperature
Sample Type:
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Elution Volume:
50–100 μL
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity:
50 Preps
Test Time:
__
Starting Material Amount:
Bacteria: ≤109cellsBlood: 0.5 mLCells: ≤107Tissue and Insects: 30 mgYeast: ≤4 x 108cellsEnzymatic reactions: ≤100 μL
Column Type: __
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: Total RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: Up to 160 μg
Content And Storage: 600 μL Proteinase K, Store at room temperature until opened, -20°C after opening, 40 mL Lysis Buffer, 40 mL Wash Buffer 1 (concentrated), 23 mL Wash Buffer 2 (concentrated), 30 mL Nuclease-free Water, 50 GeneJET RNA Spin Columns with Collection Tubes, 50 Collection Tubes (2 mL), 50 Collection Tubes (1.5 mL)
Sample Type: Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Elution Volume: 50 to 100 μL
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity: 50 Preps
Test Time: __
Starting Material Amount: __
Column Type:
__
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
Total RNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
Up to 160 μg
Content And Storage:
600 μL Proteinase K, Store at room temperature until opened, -20°C after opening, 40 mL Lysis Buffer, 40 mL Wash Buffer 1 (concentrated), 23 mL Wash Buffer 2 (concentrated), 30 mL Nuclease-free Water, 50 GeneJET RNA Spin Columns with Collection Tubes, 50 Collection Tubes (2 mL), 50 Collection Tubes (1.5 mL)
Sample Type:
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Elution Volume:
50 to 100 μL
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity:
50 Preps
Test Time:
__
Starting Material Amount:
__
Column Type: __
Isolation Technology: Silica Spin Column
Scale: Mini
Final Product Type: Total RNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: Room Temperature
Yield: Up to 160 μg
Content And Storage: • 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening• 200 mL Lysis Buffer; room temperature• 200 mL Wash Buffer 1 (concentrated); room temperature• 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Nuclease-free Water; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Sample Type: Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Elution Volume: 50–100 μL
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity: 250 Preps
Test Time: __
Starting Material Amount: Bacteria: ≤109cellsBlood: 0.5 mLCells: ≤107Tissue and Insects: 30 mgYeast: ≤4 x 108cellsEnzymatic reactions: ≤100 μL
Column Type:
__
Isolation Technology:
Silica Spin Column
Scale:
Mini
Final Product Type:
Total RNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
Room Temperature
Yield:
Up to 160 μg
Content And Storage:
• 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening• 200 mL Lysis Buffer; room temperature• 200 mL Wash Buffer 1 (concentrated); room temperature• 100 mL Wash Buffer 2 (concentrated); room temperature• 125 mL Nuclease-free Water; room temperature• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature• 250 Collection Tubes (2 mL); room temperature• 250 Collection Tubes (1.5 mL); room temperature
Sample Type:
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Elution Volume:
50–100 μL
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity:
250 Preps
Test Time:
__
Starting Material Amount:
Bacteria: ≤109cellsBlood: 0.5 mLCells: ≤107Tissue and Insects: 30 mgYeast: ≤4 x 108cellsEnzymatic reactions: ≤100 μL