A47156
Thermo Scientific™ MagMAX™ Plant DNA Isolation Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A47156-Each-of-1 | In Stock | ₹ 1,65,600.00 |
A47156 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,65,600.00
GST (18%): ₹ 29,808.00
Total Price: ₹ 1,95,408.00
Content And Storage
• 30 mL RNase A; –20°C• 30 mL DNA Binding Beads; 4°C• 625 mL Lysis Buffer A; room temperature• 88 mL Lysis Buffer B; room temperature• 172 mL Precipitation Solution; room temperature• 470 mL Wash Solution 1 (concentrated); room temperature• 315 mL Wash Solution 2 (concentrated); room temperature• 313 mL Elution Buffer; room temperature
Sample Type
Plants
Final Product Type
DNA
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity
1000 Preps
Test Time
Up to 39 min. when using automated protocol
Starting Material Amount
≤100 mg fresh≤ 20 mg dried
Isolation Technology
Magnetic Bead
Elution Volume
50 to 200 μL
For Use With (Equipment)
KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
High-throughput Compatibility
High-throughput Compatible
Shipping Condition
RNase A: Dry IceAll other components: Room Temperature
Yield
≤15 μg
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Description
- Description The MagMAX™ Plant DNA Isolation Kit is designed for automated high-throughput or manual purification of DNA from a wide variety of plant species
- The kit uses MagMAX magnetic bead technology, eliminating the need for phenol/chloroform extraction or alcohol precipitation
- The procedures are optimized for the isolation of DNA from 10–100 mg plant samples
- DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, age of tissue sample, and storage conditions
- The MagMAX Plant DNA Isolation Kit relies on magnetic bead technology, enabling reproducible yields and robust performance
- High binding capacity, uniform particle size, and rapid magnetic response of the beads makes the technology ideal for automated high-throughput nucleic acid purification, as well as for manual purification for low sample throughput
- The resulting high-quality DNA is free of proteins, nucleases, and other contaminants or inhibitors, and can be used in a wide range of downstream applications such as PCR, qPCR, or next-generation sequencing
- See bar graph below for typical genomic DNA yields from various sources
- Features of the MagMAX Plant DNA Isolation Kit include: • Versatile—suitable for most plant types and parts, including woody, polyphenol-rich, and lignified samples • High DNA yields—compared to traditional CTAB method • High quality—isolates high integrity DNA, free of inhibitors and RNA contamination • Flexible—easily adjustable for high-throughput automation platforms and manual processing Applications Isolated DNA is ideal for use in common molecular biology procedures, including: • qPCR • next-generation sequencing Benefits of MagMAX magnetic bead–based purification The magnetic bead–based purification format of the kit allows for easy scaling of the number of samples processed either manually with a magnetic stand or with a magnetic particle processor such as the KingFisher™ Duo Prime or KingFisher™ Flex processors
- Magnetic beads offer many benefits compared to other technologies for isolating nucleic acids
- Beads bind the RNA and DNA more efficiently than glass fiber filters, resulting in higher and more consistent yields
- Additionally, because filters and vacuum manifolds are not used, there is no risk of undigested tissue particulates clogging these items during the extraction process
- This clogging issue is of particular concern with fibrous plant tissue samples that that are not fully lysed during the disruption and homogenization steps
- Optimized bead surface chemistry The Dynabeads™ MyOne™ Carboxylic Acid utilized in the MagMAX Plant DNA Kit has an optimized surface chemistry with high specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture
- These aspects make Dynabeads magnetic technology ideal for the recovery of the DNA targets found in a variety of plant samples
- Once captured, the DNA is then eluted in 150 μL and ready for downstream applications.
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Content And Storage: • 30 mL RNase A; –20°C• 30 mL DNA Binding Beads; 4°C• 625 mL Lysis Buffer A; room temperature• 88 mL Lysis Buffer B; room temperature• 172 mL Precipitation Solution; room temperature• 470 mL Wash Solution 1 (concentrated); room temperature• 315 mL Wash Solution 2 (concentrated); room temperature• 313 mL Elution Buffer; room temperature
Sample Type: Plants
Final Product Type: DNA
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity: 1000 Preps
Test Time: Up to 39 min. when using automated protocol
Starting Material Amount: ≤100 mg fresh≤ 20 mg dried
Isolation Technology: Magnetic Bead
Elution Volume: 50 to 200 μL
For Use With (Equipment): KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
High-throughput Compatibility: High-throughput Compatible
Shipping Condition: RNase A: Dry IceAll other components: Room Temperature
Yield: ≤15 μg
Content And Storage:
• 30 mL RNase A; –20°C• 30 mL DNA Binding Beads; 4°C• 625 mL Lysis Buffer A; room temperature• 88 mL Lysis Buffer B; room temperature• 172 mL Precipitation Solution; room temperature• 470 mL Wash Solution 1 (concentrated); room temperature• 315 mL Wash Solution 2 (concentrated); room temperature• 313 mL Elution Buffer; room temperature
Sample Type:
Plants
Final Product Type:
DNA
For Use With (Application):
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity:
1000 Preps
Test Time:
Up to 39 min. when using automated protocol
Starting Material Amount:
≤100 mg fresh≤ 20 mg dried
Isolation Technology:
Magnetic Bead
Elution Volume:
50 to 200 μL
For Use With (Equipment):
KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
High-throughput Compatibility:
High-throughput Compatible
Shipping Condition:
RNase A: Dry IceAll other components: Room Temperature
Yield:
≤15 μg
Content And Storage: • 750 mL Lysis Buffer; room temperature• 30 mL RNA Binding Beads; 4°C• 3.4 mL DNase I; –20°C• 10.5 mL DNase I Reconstitution Buffer; –20°C• 32 mL Manganese Chloride Solution; –20°C• 125 mL DNase I Buffer (2X); –20°C• 1146 mL Wash Solution 1 (concentrated); room temperature• 521 mL Wash Solution 2 (concentrated); room temperature• 210 mL Rebinding Buffer; room temperature• 313 mL Nuclease-free Water; room temperature
Sample Type: Plant Samples
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Quantity: 1000 Preps
Test Time: __
Starting Material Amount: __
Isolation Technology: Magnetic Bead
Elution Volume: 100 μL
For Use With (Equipment): KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
High-throughput Compatibility: High-throughput Compatible
Shipping Condition: Room Temperature
Yield: ≤47 μg
Content And Storage:
• 750 mL Lysis Buffer; room temperature• 30 mL RNA Binding Beads; 4°C• 3.4 mL DNase I; –20°C• 10.5 mL DNase I Reconstitution Buffer; –20°C• 32 mL Manganese Chloride Solution; –20°C• 125 mL DNase I Buffer (2X); –20°C• 1146 mL Wash Solution 1 (concentrated); room temperature• 521 mL Wash Solution 2 (concentrated); room temperature• 210 mL Rebinding Buffer; room temperature• 313 mL Nuclease-free Water; room temperature
Sample Type:
Plant Samples
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Quantity:
1000 Preps
Test Time:
__
Starting Material Amount:
__
Isolation Technology:
Magnetic Bead
Elution Volume:
100 μL
For Use With (Equipment):
KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
High-throughput Compatibility:
High-throughput Compatible
Shipping Condition:
Room Temperature
Yield:
≤47 μg
Content And Storage: __
Sample Type: __
Final Product Type: __
For Use With (Application): __
Quantity: __
Test Time: __
Starting Material Amount: __
Isolation Technology: __
Elution Volume: 50 μL
For Use With (Equipment): __
High-throughput Compatibility: __
Shipping Condition: Room Temperature
Yield: __
Content And Storage:
__
Sample Type:
__
Final Product Type:
__
For Use With (Application):
__
Quantity:
__
Test Time:
__
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
50 μL
For Use With (Equipment):
__
High-throughput Compatibility:
__
Shipping Condition:
Room Temperature
Yield:
__
Content And Storage: RiboMinus Isolation Kits include: RiboMinus Magnetic Beads, RiboMinus human/mouse, bacteria, or yeast probe, and Hybridization BufferThe optional RiboMinus Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water.Store the isolation modules at 4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.
Sample Type: Total RNA (Bacterial)
Final Product Type: Transcriptome RNA
For Use With (Application): Next Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
Quantity: Each
Test Time: __
Starting Material Amount: 100 ng to 5 ug of total RNA
Isolation Technology: Magnetic Bead
Elution Volume: __
For Use With (Equipment): __
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Shipping Condition: __
Yield: __
Content And Storage:
RiboMinus Isolation Kits include: RiboMinus Magnetic Beads, RiboMinus human/mouse, bacteria, or yeast probe, and Hybridization BufferThe optional RiboMinus Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water.Store the isolation modules at 4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.
Sample Type:
Total RNA (Bacterial)
Final Product Type:
Transcriptome RNA
For Use With (Application):
Next Generation Sequencing, Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
Quantity:
Each
Test Time:
__
Starting Material Amount:
100 ng to 5 ug of total RNA
Isolation Technology:
Magnetic Bead
Elution Volume:
__
For Use With (Equipment):
__
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Shipping Condition:
__
Yield:
__