Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit

Description

• The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane
• In as little as 90 minutes, purify up to 1.5 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering
• Advantages of the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit include: • Simple and fast protocol—purification typically takes 90 minutes • High yield—isolate up to 1.5 mg of high quality plasmid DNA • Endotoxin-free plasmid—less than 0.1 EU/μg, ideal for sensitive applications • High purity—ultralow levels of protein, RNA, and genomic DNA contamination • Compatibility—suitable for use with ExpiCHO and Expi293 expression systems • Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin Simple and fast protocol Unlike other anion-exchange-based endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification
• There is no waiting for gravity-flow columns and the entire purification typically takes 90 minutes (including the final precipitation step)
• Endotoxin-free plasmid Purify endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines in vivo
• Compatible with ExpiCHO and Expi293 expression systems Plasmid purified using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit is fully compatible with the Gibco ExpiCHO and Expi293 transient mammalian expression systems
• Superior quality with endotoxin-free components Rest assured that the kit is of high quality and has additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind.