K210011
Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K210011-Each-of-1 | In Stock | ₹ 40,296.00 |
K210011 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,296.00
GST (18%): ₹ 7,253.28
Total Price: ₹ 47,549.28
Concentration
20 mg/mL
Content And Storage
• Resuspension buffer• RNase A (20 mg/mL)• Lysis buffer• Precipitation buffer• Wash buffers• TE buffer• Spin columns in wash tubes• Elution tubes
Isolation Technology
Silica Spin Column
Scale
Mini
For Use With (Application)
Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
No. of Reactions
250 Preps
Product Line
PureLink™
Quantity
250 Preps
Target
Plasmid DNA
Column Type
Spin Column
Format
Spin Column
Sample Type
Bacterial Culture
Final Product Type
Plasmid DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Prep Scale
< 100 μg (Small-Scale) Plasmid DNA
Product Type
Quick Plasmid MiniPrep Kit
Shipping Condition
Room Temperature
Test Time
30 to 45 min.
Related Products
Description
- Description The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns
- Features of the PureLink™ Quick Plasmid Miniprep Kit: Fast —ready-to-use plasmid DNA typically in about 30 min Flexible —formats work with vacuum and centrifugation platforms Reliable and cost-effective isolation of plasmid DNA The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30–45 min, using the PureLink™ spin columns
- These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA
- Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA
- Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer
- The eluted DNA is suitable for all routine downstream applications
- Compatible with flexible formats The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA
- Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A 260 /A 280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications
- Isolated DNA can be used for a variety of downstream applications Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR
- Order Info Shipping Condition: Room Temperature
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Concentration: 20 mg/mL
Content And Storage: • Resuspension buffer• RNase A (20 mg/mL)• Lysis buffer• Precipitation buffer• Wash buffers• TE buffer• Spin columns in wash tubes• Elution tubes
Isolation Technology: Silica Spin Column
Scale: Mini
For Use With (Application): Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
No. of Reactions: 250 Preps
Product Line: PureLink™
Quantity: 250 Preps
Target: Plasmid DNA
Column Type: Spin Column
Format: Spin Column
Sample Type: Bacterial Culture
Final Product Type: Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
Product Type: Quick Plasmid MiniPrep Kit
Shipping Condition: Room Temperature
Test Time: 30 to 45 min.
Concentration:
20 mg/mL
Content And Storage:
• Resuspension buffer• RNase A (20 mg/mL)• Lysis buffer• Precipitation buffer• Wash buffers• TE buffer• Spin columns in wash tubes• Elution tubes
Isolation Technology:
Silica Spin Column
Scale:
Mini
For Use With (Application):
Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
No. of Reactions:
250 Preps
Product Line:
PureLink™
Quantity:
250 Preps
Target:
Plasmid DNA
Column Type:
Spin Column
Format:
Spin Column
Sample Type:
Bacterial Culture
Final Product Type:
Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Prep Scale:
< 100 μg (Small-Scale) Plasmid DNA
Product Type:
Quick Plasmid MiniPrep Kit
Shipping Condition:
Room Temperature
Test Time:
30 to 45 min.
Concentration: __
Content And Storage: • 2 x 90 mL Gel Solubilization Buffer L3; room temperature• 16 mL Wash Buffer W1; room temperature• 15 mL Elution Buffer E5; room temperature• 50 Quick Gel Extraction Columns with Collection Tubes; room temperature• 50 Recovery Tubes; room temperature
Isolation Technology: Silica Spin Column
Scale: __
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
No. of Reactions: __
Product Line: __
Quantity: 50 Preps
Target: __
Column Type: __
Format: __
Sample Type: Agarose Gels, Gel Samples
Final Product Type: DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: __
Product Type: __
Shipping Condition: Room Temperature
Test Time: 30 min.
Concentration:
__
Content And Storage:
• 2 x 90 mL Gel Solubilization Buffer L3; room temperature• 16 mL Wash Buffer W1; room temperature• 15 mL Elution Buffer E5; room temperature• 50 Quick Gel Extraction Columns with Collection Tubes; room temperature• 50 Recovery Tubes; room temperature
Isolation Technology:
Silica Spin Column
Scale:
__
For Use With (Application):
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
No. of Reactions:
__
Product Line:
__
Quantity:
50 Preps
Target:
__
Column Type:
__
Format:
__
Sample Type:
Agarose Gels, Gel Samples
Final Product Type:
DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Prep Scale:
__
Product Type:
__
Shipping Condition:
Room Temperature
Test Time:
30 min.
Concentration: __
Content And Storage: The PureLink™ Nucleic Acid Purification Rack includes:• Column Holder Rack (for processing 12 miniprep, 8 midiprep, and 4 maxiprep columns)• Collection Tube Rack (capable of accommodating various types and sizes of recovery tubes)• Large Capacity Waste Tray (for collecting waste)
Isolation Technology: __
Scale: __
For Use With (Application): __
No. of Reactions: __
Product Line: PureLink™
Quantity: 1 Ea.
Target: __
Column Type: __
Format: Rack
Sample Type: __
Final Product Type: __
High-throughput Compatibility: __
Prep Scale: __
Product Type: Nucleic Acid Purification Rack
Shipping Condition: Room Temperature
Test Time: __
Concentration:
__
Content And Storage:
The PureLink™ Nucleic Acid Purification Rack includes:• Column Holder Rack (for processing 12 miniprep, 8 midiprep, and 4 maxiprep columns)• Collection Tube Rack (capable of accommodating various types and sizes of recovery tubes)• Large Capacity Waste Tray (for collecting waste)
Isolation Technology:
__
Scale:
__
For Use With (Application):
__
No. of Reactions:
__
Product Line:
PureLink™
Quantity:
1 Ea.
Target:
__
Column Type:
__
Format:
Rack
Sample Type:
__
Final Product Type:
__
High-throughput Compatibility:
__
Prep Scale:
__
Product Type:
Nucleic Acid Purification Rack
Shipping Condition:
Room Temperature
Test Time:
__
Concentration: __
Content And Storage: The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.
Isolation Technology: Anion Exchange Resin
Scale: __
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions: 25 Preps
Product Line: PureLink™
Quantity: 25 Preps
Target: Plasmid DNA
Column Type: Filter Column
Format: Column
Sample Type: Bacterial Culture
Final Product Type: Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
Product Type: Plasmid Filter MidiPrep Kit
Shipping Condition: Room Temperature
Test Time: 90 min.
Concentration:
__
Content And Storage:
The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.
Isolation Technology:
Anion Exchange Resin
Scale:
__
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
No. of Reactions:
25 Preps
Product Line:
PureLink™
Quantity:
25 Preps
Target:
Plasmid DNA
Column Type:
Filter Column
Format:
Column
Sample Type:
Bacterial Culture
Final Product Type:
Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Prep Scale:
100-200 μg (Medium-Scale) Plasmid DNA
Product Type:
Plasmid Filter MidiPrep Kit
Shipping Condition:
Room Temperature
Test Time:
90 min.