Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit

Description

• The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns
• Features of the PureLink™ Quick Plasmid Miniprep Kit: Fast —ready-to-use plasmid DNA typically in about 30 min Flexible —formats work with vacuum and centrifugation platforms Reliable and cost-effective isolation of plasmid DNA The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30–45 min, using the PureLink™ spin columns
• These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA
• Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA
• Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer
• The eluted DNA is suitable for all routine downstream applications
• Compatible with flexible formats The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA
• Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A 260 /A 280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications
• Isolated DNA can be used for a variety of downstream applications Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR
• Order Info Shipping Condition: Room Temperature