Invitrogen™ BaculoDirect™ C-Term Transfection Kit

Description

• Engineered to include attR sites for quick and efficient recombination with Gateway entry clone Gene of interest is recombined from entry clone into BaculoDirect Linear DNA using simple, one-hour LR reaction Resulting reaction mix contains recombinant baculovirus carrying gene of interest and is used directly to transfect insect cells Eliminates need for transforming bacteria and isolating large bacmid or co-transfection of transfer vector and linear baculovirus DNA into insect cells togreatly reduce hands-on time Purified baculovirus can be isolated in less than one week Gateway Linear DNA: Designed for rapid cloning with Gateway entry clone and subsequent expression in Sf9 or Sf21 insect cells Strong polyhedrin promoter for high-level expression R sites for efficient recombination with attL-flanked Gateway entry vector TK gene for negative selection using ganciclovir N-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect N-Term Linear DNA) TEV protease cleavage site for removal of V5-His tag following purification (BaculoDirect N-Term Linear DNA) C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect C-Term Linear DNA) Baculovirus Production and Titering, Cloning, Gateway Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis