Promega pGEM™ Vectors

Description

• Multiple cloning site provides a selection of restriction sites and is flanked by the SP6 and T7 RNA polymerase promoters lac Z α-peptide and multiple cloning site arrangement from pUC18 allow recombinants to be selected using blue/white screening pGEM-3Z and -4Z Vectors† Versatile vectors can be used for standard cloning and in vitro transcription pGEM-3Z and pGEM-4Z vectors are identical except for the orientation of the SP6 and T7 promoters pGEM-3Zf(+/-), -5Zf(+/-), -7Zf(+/-), -9Zf(-), -11Zf(+/-), and -13Zf(+) Vectors† Vectors are derived from the pGEM-3Z vector and contain the origin of replication of the filamentous phage f1 Versatile vectors can be used for standard cloning, ssDNA production, and in vitro transcription (+) and (-) vectors with the same numeric designation are identical except for the orientation of the f1 origin pGEM-5Zf(+/-) and -7Zf(+/-) contain restriction site arrangements designed to generate unidirectional deletions with the Erase-a-Base™ System pGEM-9Zf(-) permits the excision of an insert containing the SP6 and T7 polymerase promoters