PR-P2411
Promega pGEM™ Vectors
Manufacturer: Fischer Scientific
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Promoter
SP6, T7
Content And Storage
-30°C to -10°C
Vector
pGEM-11Zf(+) vector
Restriction Site
SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII
Quantity
20 μg
For Use With (Application)
Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening
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Description
- Multiple cloning site provides a selection of restriction sites and is flanked by the SP6 and T7 RNA polymerase promoters lac Z α-peptide and multiple cloning site arrangement from pUC18 allow recombinants to be selected using blue/white screening pGEM-3Z and -4Z Vectors† Versatile vectors can be used for standard cloning and in vitro transcription pGEM-3Z and pGEM-4Z vectors are identical except for the orientation of the SP6 and T7 promoters pGEM-3Zf(+/-), -5Zf(+/-), -7Zf(+/-), -9Zf(-), -11Zf(+/-), and -13Zf(+) Vectors† Vectors are derived from the pGEM-3Z vector and contain the origin of replication of the filamentous phage f1 Versatile vectors can be used for standard cloning, ssDNA production, and in vitro transcription (+) and (-) vectors with the same numeric designation are identical except for the orientation of the f1 origin pGEM-5Zf(+/-) and -7Zf(+/-) contain restriction site arrangements designed to generate unidirectional deletions with the Erase-a-Base™ System pGEM-9Zf(-) permits the excision of an insert containing the SP6 and T7 polymerase promoters
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Promoter: SP6, T7
Content And Storage: -30°C to -10°C
Vector: pGEM-11Zf(+) vector
Restriction Site: SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII
Quantity: 20 μg
For Use With (Application): Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening
Promoter:
SP6, T7
Content And Storage:
-30°C to -10°C
Vector:
pGEM-11Zf(+) vector
Restriction Site:
SfiI, SacI, EcoRI, SalI, XhoI, BamHI, ApaI, XbaI, NotI, SphI, NsiI and HindIII
Quantity:
20 μg
For Use With (Application):
Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening
Promoter: __
Content And Storage: -30°C to -10°C
Vector: __
Restriction Site: __
Quantity: 10,000 U
For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Promoter:
__
Content And Storage:
-30°C to -10°C
Vector:
__
Restriction Site:
__
Quantity:
10,000 U
For Use With (Application):
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Promoter: __
Content And Storage: <-65°C
Vector: __
Restriction Site: __
Quantity: 500 μL
For Use With (Application): __
Promoter:
__
Content And Storage:
<-65°C
Vector:
__
Restriction Site:
__
Quantity:
500 μL
For Use With (Application):
__
Promoter: __
Content And Storage: <-65°C
Vector: __
Restriction Site: __
Quantity: 500 μL
For Use With (Application): For the high level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter
Promoter:
__
Content And Storage:
<-65°C
Vector:
__
Restriction Site:
__
Quantity:
500 μL
For Use With (Application):
For the high level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter