3623198
Promega HaloTag™ Vectors
A variety vectors for mammalian,E. coli, and cell-free protein expression
Manufacturer: Fischer Scientific
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Content And Storage
-30°C to -10°C
For Use With (Application)
Cell imaging of Protein localization or trafficking in conjunction with the fluorescent HaloTag™ ligands by using expression of HaloTag™ fusion Proteins in E. coli and cell-free Protein expression
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Description
- All vectors are compatible with Promega Flexi Vector System, directional cloning method for protein-coding sequences Vectors designated “pFC” are configured to append HaloTag 7 to carboxy-terminus of protein fusion partner Vectors designated “pFN” are configured to append HaloTag 7 to amino-terminus of protein fusion partner Vectors for Mammalian Protein Expression Vectors contain either full-length (pFC14, pFN21) or modified (pFC15, pFC16, pFC17, pFN22, pFN23, pFN24) human cytomegalovirus (CMV) promoter The modified CMV promoter, which contains various deletions, provides varying levels of protein expression levels, depending on protein of interest and cell type Vectors for E
- coli and Cell-Free Protein Expression pFN18 vectors provide T7 RNA polymerase-driven protein expression in E
- coli or in cell-free translation systems pFN19 vectors provide T7 RNA polymerase-driven protein expression in E
- coli ( ∽two- to four-fold lower protein expression compared to pFN18 vectors) or T7 or SP6 RNA polymerase-driven protein expression in cell-free translation systems pFC20 vectors provide T7 RNA polymerase-driven protein expression in E
- coli or T7 or SP6 RNA polymerase-driven protein expression in cell-free translation systems Cellular imaging, Protein:protein interaction, Protein:DNA interaction.
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Content And Storage: -30°C to -10°C
For Use With (Application): Cell imaging of Protein localization or trafficking in conjunction with the fluorescent HaloTag™ ligands by using expression of HaloTag™ fusion Proteins in E. coli and cell-free Protein expression
Content And Storage:
-30°C to -10°C
For Use With (Application):
Cell imaging of Protein localization or trafficking in conjunction with the fluorescent HaloTag™ ligands by using expression of HaloTag™ fusion Proteins in E. coli and cell-free Protein expression
Content And Storage: __
For Use With (Application): __
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For Use With (Application):
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For Use With (Application): DNA Sequencing
Content And Storage:
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For Use With (Application):
DNA Sequencing
Content And Storage: __
For Use With (Application): DNA Sequencing
Content And Storage:
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For Use With (Application):
DNA Sequencing