4925855
Invitrogen™ pBAD TOPO™ TA Expression Kit
Specifically designed for one-step cloning and regulated prokaryotic expression ofTaq-amplified PCR products
Manufacturer: Fischer Scientific
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Constitutive or Inducible System
Inducible
Promoter
araBAD
Selection Agent (Eukaryotic)
None
Bacterial or Yeast Strain
LMG194, TOP10
Expression System
E. coli
Cloning Method
TOPO-TA
Product Line
TOPO™
Inducing Agent
Arabinose
Product Type
TA Expression Kit
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Expression Mechanism
Cell-Based Expression
Vector
pBAD
Related Products
Description
- Linearized, topoisomerase I-activated vector for 5-minute cloning of Taq amplified PCR products araBAD promoter for tightly regulated expression in E
- coli V5 epitope tag for detection with Anti-V5 Antibody C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody Enterokinase cleavage site for removal of N-terminal leader peptide Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
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Constitutive or Inducible System: Inducible
Promoter: araBAD
Selection Agent (Eukaryotic): None
Bacterial or Yeast Strain: LMG194, TOP10
Expression System: E. coli
Cloning Method: TOPO-TA
Product Line: TOPO™
Inducing Agent: Arabinose
Product Type: TA Expression Kit
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Expression Mechanism: Cell-Based Expression
Vector: pBAD
Constitutive or Inducible System:
Inducible
Promoter:
araBAD
Selection Agent (Eukaryotic):
None
Bacterial or Yeast Strain:
LMG194, TOP10
Expression System:
E. coli
Cloning Method:
TOPO-TA
Product Line:
TOPO™
Inducing Agent:
Arabinose
Product Type:
TA Expression Kit
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Cleavage:
EK (Enterokinase) Recognition Site
Expression Mechanism:
Cell-Based Expression
Vector:
pBAD
Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Bacterial or Yeast Strain: __
Expression System: __
Cloning Method: Directional TOPO
Product Line: TOPO™, pcDNA™
Inducing Agent: __
Product Type: TOPO Expression Kit
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Vector: Directional TOPO Vectors, pcDNA
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Bacterial or Yeast Strain:
__
Expression System:
__
Cloning Method:
Directional TOPO
Product Line:
TOPO™, pcDNA™
Inducing Agent:
__
Product Type:
TOPO Expression Kit
Antibiotic Resistance Bacterial:
__
Cleavage:
__
Expression Mechanism:
__
Vector:
Directional TOPO Vectors, pcDNA
Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Bacterial or Yeast Strain: __
Expression System: __
Cloning Method: TOPO-TA
Product Line: TOPO™, pcDNA™
Inducing Agent: __
Product Type: TOPO TA Expression Kit
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Vector: TOPO-TA Vectors, pcDNA
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Bacterial or Yeast Strain:
__
Expression System:
__
Cloning Method:
TOPO-TA
Product Line:
TOPO™, pcDNA™
Inducing Agent:
__
Product Type:
TOPO TA Expression Kit
Antibiotic Resistance Bacterial:
__
Cleavage:
__
Expression Mechanism:
__
Vector:
TOPO-TA Vectors, pcDNA
Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Bacterial or Yeast Strain: __
Expression System: __
Cloning Method: TOPO-TA
Product Line: TOPO™, pcDNA™
Inducing Agent: __
Product Type: TOPO TA Expression Kit
Antibiotic Resistance Bacterial: __
Cleavage: __
Expression Mechanism: __
Vector: TOPO-TA Vectors, pcDNA
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Selection Agent (Eukaryotic):
Geneticin™ (G-418)
Bacterial or Yeast Strain:
__
Expression System:
__
Cloning Method:
TOPO-TA
Product Line:
TOPO™, pcDNA™
Inducing Agent:
__
Product Type:
TOPO TA Expression Kit
Antibiotic Resistance Bacterial:
__
Cleavage:
__
Expression Mechanism:
__
Vector:
TOPO-TA Vectors, pcDNA