V45001

Invitrogen™ pBAD/gIII Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 V45001-Each-of-1 In Stock ₹ 1,03,960.00

V45001 - Each of 1

₹ 1,03,960.00

In Stock

Quantity

1

Base Price: ₹ 1,03,960.00

GST (18%): ₹ 18,712.80

Total Price: ₹ 1,22,672.80

Constitutive or Inducible System

Inducible

Promoter

araBAD

Selection Agent (Eukaryotic)

None

Antibiotic Resistance Bacterial

Ampicillin (AmpR)

Cloning Method

Restriction Enzyme/MCS

Vector

pBAD

Inducing Agent

Arabinose

Product Type

Bacterial Expression Vector

Content And Storage

The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10E. colistabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.

Protein Tag

c-Myc Epitope Tag

Quantity

1 kit

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Description

  • Description To simplify purification, vector pBAD/gIII encodes a leader peptide which directs recombinant protein into the periplasmic space Features araBAD promoter for tightly regulated expression Translation initiation signals for optimized E
  • coli expression Leader peptide from bacteriophage fd gene III protein (gIII) for secreted expression C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin C-terminal c-myc epitope for detection and analysis with an Anti- myc Antibody A set of three vectors is provided (A, B, and C): Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of the gene Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

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Invitrogen™

V45001

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Constitutive or Inducible System:
Inducible

Promoter:
araBAD

Selection Agent (Eukaryotic):
None

Antibiotic Resistance Bacterial:
Ampicillin (AmpR)

Cloning Method:
Restriction Enzyme/MCS

Vector:
pBAD

Inducing Agent:
Arabinose

Product Type:
Bacterial Expression Vector

Content And Storage:
The pBAD/gIII Kit includes 20 μg each of pBAD/gIII A, B, & C, 20 μg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10E. colistabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.

Protein Tag:
c-Myc Epitope Tag

Quantity:
1 kit

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Constitutive or Inducible System:
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Promoter:
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The pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit contains 6 μg of vector and 10 μg of pSCREEN-iT™/lacZ-GW/CDK2 control vector, the positive control Stealth™RNAilacZ, a negative control Scrambled Stealth RNAi™ siRNA, and 1X annealing/dilution buffer. Store at -20°C. The BLOCK-iT™ RNAi Target Screening Kit contains the pSCREEN-iT™/lacZ-DEST Gateway™ Vector Kit, 250 μl of Lipofectamine™ 2000 Reagent, and the FluoReporter™lacZ/Galactosidase Quantification Kit. Store the Lipofectamine™ 2000 Reagent at +4°C. Store the FluoReporter™ Kit at -20°C protected from light. The BLOCK-iT™ RNAi Target Screening System contains the BLOCK-iT™ RNAi Target Screening Kit, LR Clonase™ enzyme mix, and pCR ¤ 8/GW/TOPO ¤ TA Cloning Kit. Store the LR Clonase™ enzyme mix at -80°C. The pCR™ 8/GW/TOPO™ TA Cloning Kit includes the ready-to-use pCR™ 8/GW/TOPO™ vector, dNTPs, salt solution, water, forward and reverse sequencing primers, control template and PCR primers, One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a pUC19 control plasmid. Store the Competent Cells at -80°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.

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Quantity:
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