4925865

Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit

Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression

Manufacturer: Fischer Scientific

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Constitutive or Inducible System

Constitutive

Promoter

CMV

Selection Agent (Eukaryotic)

Geneticin™ (G-418)

Cloning Method

Directional TOPO

Product Line

TOPO™, pcDNA™

Delivery Type

Transfection

Product Type

TOPO Expression Kit

Protein Tag

His Tag (6x), V5 Epitope Tag

Vector

Directional TOPO Vectors, pcDNA

For Use With (Application)

Constitutive Expression

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Description

  • Proofreading enzyme is used: fewer errors in cloned genes Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation CMV promoter: high expression levels C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody 6xHis tag for rapid protein purification on nickel-chelating resin Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Dry Ice

Compare Similar Items

Show Difference

Img

Fischer Scientific

4925865

Linearized, topoisomerase I-activat...


Constitutive or Inducible System:
Constitutive

Promoter:
CMV

Selection Agent (Eukaryotic):
Geneticin™ (G-418)

Cloning Method:
Directional TOPO

Product Line:
TOPO™, pcDNA™

Delivery Type:
Transfection

Product Type:
TOPO Expression Kit

Protein Tag:
His Tag (6x), V5 Epitope Tag

Vector:
Directional TOPO Vectors, pcDNA

For Use With (Application):
Constitutive Expression

Img

Fischer Scientific

4925873

Provides efficient, 5-minute, one-s...


Constitutive or Inducible System:
Constitutive

Promoter:
CMV

Selection Agent (Eukaryotic):
Geneticin™ (G-418)

Cloning Method:
TOPO-TA

Product Line:
TOPO™, pcDNA™

Delivery Type:
Transfection

Product Type:
TOPO TA Expression Kit

Protein Tag:
His Tag (6x), V5 Epitope Tag

Vector:
TOPO-TA Vectors, pcDNA

For Use With (Application):
Constitutive Expression

Img

Fischer Scientific

4925873

Provides efficient, 5-minute, one-s...


Constitutive or Inducible System:
Constitutive

Promoter:
CMV

Selection Agent (Eukaryotic):
Geneticin™ (G-418)

Cloning Method:
TOPO-TA

Product Line:
TOPO™, pcDNA™

Delivery Type:
Transfection

Product Type:
TOPO TA Expression Kit

Protein Tag:
His Tag (6x), V5 Epitope Tag

Vector:
TOPO-TA Vectors, pcDNA

For Use With (Application):
Constitutive Expression

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Fischer Scientific

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Recombinant preparation of the cata...


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Promoter:
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Selection Agent (Eukaryotic):
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Cloning Method:
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Product Line:
EKMax™

Delivery Type:
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Protein Tag:
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