Invitrogen™ pcDNA™3.1/V5-His TOPO™ TA Expression Kit

Description

• No ligase, post-PCR procedures, or PCR primers containing specific sequences are required Once cloned, analyzed, and transfected, PCR product will express directly in mammalian cell lines pcDNA3.1/V5-His TOPO vector features strong CMV promoter for high-level, constitutive expression C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with Anti-His (C-term)antibody Topoisomerase activation of pcDNA3.1/V5-His-TOPO vector allows PCR products to be ligated in 5 minutes on bench top and results in 90% recombinants Vector features strong CMV promoter for high-level, constitutive expression C-terminal V5 epitope tag for efficient detection of recombinant proteins with Anti-V5 antibody Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis Order Info Shipping Condition: Dry Ice