Description Recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves peptide bond after lysine residue Enzyme can be used to cleave any fusion protein that carries this peptide sequence Well-suited to removal of fusion tags from recombinant proteins Unit Definition: One unit of EKMaxis amount of enzyme required to digest 20μg of thioredoxin-CAT fusion protein to 90% completion in 16 hours at 37°C; One EKMaxunit is equivalent to ∽190 trypsinogen activation units GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis