6921051

MilliporeSigma™ Novagen™ pSTBlue-1 AccepTor™ Vector Giga Cloning Kit

Features NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. MilliporeSigma™; Novagen™ pSTBlue-1 AccepTor™ Vector Giga Cloning Kit is designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3'-dA nucleotide overhangs.

Manufacturer: Fischer Scientific

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Product Type

pSTBlue-1 Vector Giga Cloning Kit

Content And Storage

Store at ≤70°C.

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Description

  • Vector enables direct ligation by virtue of single 3'-dU overhangs that anneal with 3'-dA extensions on PCR products, the dU residues are converted to dT residues in vivo No restriction enzymes or special primers Direct ligation of PCR product with vector Compatible with polymerases that leave single 3´-dA overhangs Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors Simple protocol takes as little as 40 minutes from PCR product to plating transformants

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