70-635-3

MilliporeSigma™ Introductory pETBlue-2 Perfectly Blunt Cloning Kit

Manufacturer: MilliporeSigma™

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Pack Size SKU Availability Price
Each of 1 70-635-3-Each-of-1 In Stock ₹ 28,021.76

70-635-3 - Each of 1

₹ 28,021.76

In Stock

Quantity

1

Base Price: ₹ 28,021.76

GST (18%): ₹ 5,043.917

Total Price: ₹ 33,065.677

Content And Storage

Store at -70°C.

Quantity

100 Reactions

For Use With (Application)

Cloning of DNA generated by PCR; Cloning restriction fragments, cDNA, or sheared DNA

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Description

  • Description The Perfectly Blunt™ Cloning Kits are designed for simplified cloning of DNA generated by PCR using any type of DNA polymerase
  • This approach enables the use of high-fidelity proofreading enzymes for amplification, thus decreasing the probability of generating mutations in the target sequence
  • In addition, under many conditions blunt cloning is more efficient than T-cloning, most likely due to the observation that the efficiency of single dA addition by Taq DNA polymerase varies significantly depending on the sequence context of the DNA ends, and even the number of PCR cycles performed.

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