MilliporeSigma™ pET-44 Ek/LIC Vector Kit

Description

• The pET-44 vectors incorporate the Nus·Tag™ technology, which allows target proteins to be expressed as fusions to the highly soluble NusA protein
• According to a modeling study, the NusA protein has the highest potential for solubility among more than 4000 E
• coli proteins, and therefore increases the proportion of soluble protein when expressed in E
• coli as a fusion partner
• Ek/LIC represents a highly flexible cloning strategy in which a single insert preparation can be simultaneously cloned into a wide variety of compatible Ligation Independent Cloning (LIC) vectors to allow high-throughput optimization of target protein expression.